Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3691
標題: 利用數值模型定量尿素經皮吸收之滲透係數
Quantification of Porcine Skin Diffusivity in Transdermal Diffusion with a Numerical Model
作者: 吳信賢
Wu, Shin-Shian
關鍵字: Transdermal
尿素
Permeability
Urea
In vitro
滲透係數
數值模型
出版社: 化學工程學系所
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摘要: 藥物經皮輸送系統為新的藥物傳遞應用方法,目前藥物經皮輸送系統發展中,皮膚表皮的角質層被視為藥物經皮滲透最大的阻礙,為了降低藥物分子傳遞的阻礙與提高經皮吸收的效率,針對角質層的結構與特性加以研究,發展促進藥物滲透的方法,其中包含物理性與化學性等多類型的方法。 本篇研究以尿素溶液為藥物分子,在體外模式進行藥物滲透豬皮的全皮組織實驗,而尿素屬於化學性促滲劑之ㄧ,促滲劑主要作用於角質層,藉由改變角質細胞及細胞間脂質的組成與結構,使藥物分子更容易穿透角質層深入皮下。尿素溶液加入尿素分解酶反應後,可以利用簡單快速的電導度法進行定量分析,測得溶液電導度後經由標準曲線求得尿素溶液濃度。實驗裝置使用垂直式Franz Diffusion Cell(FDC)與水平式Side by Side Diffusion Cell(SDC)兩種滲透儀器,比較體外滲透實驗下完整的全皮樣品,尿素經皮滲透的結果。 實驗結果以尿素累積滲透量對實驗時間作圖,觀察尿素累積滲透的情形,再加入數值模型量化尿素滲透全皮組織的表觀滲透係數,結果FDC與SDC的滲透係數分別為6.1×10-6cm/s與6.9×10-6cm/s,與相關研究文獻進行比對與討論,除了不同藥物分子的莫爾體積會影響滲透係數,仍有許多因素會影響藥物分子對皮膚的滲透係數,都是未來必須進行研究與確認尿素經皮滲透的模式與皮膚的滲透係數。
Transdermal Diffusion System (TDDS) finds a variety of applications in drug delivery nowadays and is viewed a potential method in controlled release of drug. Currently, it is widely accepted that stratum corneum(SC) composes the major diffusional resistance in transdermal diffusion process. Therefore it demands elucidation on structure and characteristics of SC in order to improve the efficacy of drug delivery with physical and chemical protocols. Commonly known as chemical enhancer in TDDS, urea alters the structure and composition of corneocyte and intracellular lipids, and thus makes it easier for molecules to penetrate through stratum corneum. In this research, however, urea itself is employed as a tracer to diffuse through whole porcine skin in vitro. The diffusion process is recorded as the conductivity variation in time course. Concentration of urea is easily converted from its conductivity with aid of calibration curves predetermined. Experimental results show the accumulation of tracer quantity diffused through the skin sample as time proceeds. In parallel, a simple diffusion model is developed, attempting to describe this mass transport process in mathematical point of view. Output of the comparison between model data and experimental results generates the apparent diffusivity of the whole skin, which quantify the molecular mobility through the skin. Results are 6.110-6cm/s and 6.910-6cm/s for diffusions in vertical and horizontal cells respectively. The former is validated against literature data(210-7cm/s) and discussed from molecular viewpoint theoretically. This study builds up a quantitative base for the upcoming researches aiming at improvement of TDDS.
URI: http://hdl.handle.net/11455/3691
其他識別: U0005-1808200816233200
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-1808200816233200
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