Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/36956
標題: 番椒雄不稔系統鑑定及甜椒(Capsicum annuum L.)細胞質雄不稔導入之研究
Studies on the male sterile systems and the conversion from GMS to CMS in bell pepper (Capsium annuum L.)
作者: 牧立
Mulyantoro
關鍵字: Capsicum annuum L.
番椒
male sterility
Conversion from GMS to CMS
Complementation test
甜椒
雄不稔
細胞質雄不稔導入
互補試驗
出版社: 農藝學系所
引用: Andrews J (1990) Pepper: The domesticated Capsicums. Pp.1-41, Univ. of Texas Press, Austin, TX. Besset MJ (1986) Breeding vegetable crop. AVI Publising Company, Inc. Westport, Connecticut pp: 584 Cho YH (1989) Studies on male-sterile phenomena of vegetable species. PhD. Thesis Yungnam Univ., Daegu DeWitt D and Bosland PW (1993) The pepper garden. Pp. 1-22. Ten Speed Press, Berkley, CA. Frankel R, Galun E (1977) Pollination Mechanism, Reproduction and Plant breeding. Springer-Verlag Berlin Heidelberg New York 281 pp Kim DS (2005) Development RAPD and AFLP markers linked to fertility restorer (Rf) gene in chili pepper (Capsicum annuum L.). Thesis, Seoul National University Lee JD (2003) Development of AFLP Marker Linked to Gene Responsible for Stable Fertility in Cytoplasmic-Genic Male Sterility of Chili Pepper (Capsicum annuum L.). Master Thesis Department of Horticultural Science The Graduate School of Seoul National University. 44 pp Levings CS, Vasil IK (1995) The molecular biology of plant mitochondria. Kluwer, Dordrecht Yu IW, 1990. The inheritance of male sterility and its utilization for breeding in pepper (Capsicum spp.). Kyung Hee University, South Korea. PhD thesis, p. 1- 70 Abad AR, Mehrtens BJ, Mackenzie SA (1995) Specific expression in reproductive tissue and fate of a mitochondrial sterility associated protein in cytoplasmic male sterile bean. Plant Cell 7:271-285 Baoxi Z, Sanwen H, Guimei Y, Jiazhen G (2000) Two RAPD markers linked to a major fertility restoration gene in pepper. Euphytica 113:155-161 Bayles MB, Verhalen LM, McCall LL, Johnson WM, Barnes BR (2005) Recovery of recurrent parent traits when backcrossing in cotton. Crop Sci 45:2087-2095 Bellaoui M, Grelon MG, Pelletier G, Budar F (1999) The restorer Rfo gene act post transcriptionally on the stability of the ORF138 Ogura CMS-associated protein in reproductive tissue of rapeseed cybrid. Plant Mol. Biol. 40: 893-902. Bentolila S, Zethof J, Gerats T, Hanson MR (1998) Locating the petunia Rf gene on a 650-kb DNA fragment. Theor Appl Genet 96:980-988 Blum E, Liu K, Mazourek M, Yoo EY, Jahn MM, Paran I (2002) Molecular mapping of the C locus for presence of pungency in Capsicum. Genome 45:702-705 Budar F, Pelletier G (2001) Male sterility in plants: occurrence, determination, significance and use. Life Sci. 324:543-550 Connett MB, Hanson MR (1990) Differential mitochondrial electron transport through the cyanide-sensitive and cyanide-insensitive pathways in isonuclear lines of cytoplasmic male sterile, male fertile and restored Petunia. Plant Physiol 93:1634-1640 Conley CA, Hanson MR (1995) How do alteration in plant mitochondrial genome disrupt pollen development? J. Bioneg. Biomemb. 27:447-457 Curry J, Aluru M, Melendez M, Nevarez J, Melendez M, O'Connell MA (1999) Transcripts for possible capsaicinoid biosynthetic genes are differentially accumulated in pungent and non pungent. Plant Sci 148:47-57 Edwardson JR (1970) Cytoplasmic male sterility. Bot. Rev. 36:341-420 Evenor D, Guri A, Izhar S (1984) The relationship between a nuclear restorer gene and the nuclear gene for male sterility in Petunia. Theor. Appl. Genet. 67: 475-478 Gulyas G, Pakodzi K, Lee JS, Hirata Y (2006) Analysis of fertility restoration by using cytoplasmic red pepper (Capsicum annuum L.) lines. Breed Sci 56:331- 334. Hanson WD (1959) Early generation analysis of length of heterozygous chromosome segments around a locus held heterozygous with backcrossing or selfing. Genetics 44:833-837. Hanson MR, Bentolila S (2004) Interaction of mitochondrial and nuclear genes that affect male gametophyte development. Plant Cell 16:1163-1180. Hospital F (2005) Selection in backcross programmes. Phil. Trans. R. Soc. 360: 1503-1511 Jan CC, Vick BA (2007) Inheritance and allelic relationships of fertility restoration genes for seven new sources of male-sterile cytoplasm in sunflower. Plant Breed 126: 213-217 Janska H, Mackenzie SA(1993) Unusual mitochondrial genome organization in cytoplasmic male sterile common bean and the nature of cytoplasmic reversion to fertility. Genetics 135:869-879 Jo YD, Kim YM, Park MN, Yoo JH, Park MK, Kim BD, Kang BC (2009) Development and evaluation of broadly applicable markers for Restorer-of-fertility in pepper. Mol Breeding published online: 30 July 2009. DOI 10.1007/s11032-009-9318-3. Kaul MLH (1988) Male sterility in higher plants. Springer-verlag, Berlin Kempken F, Pring DR (1999) Male sterility in higher plant-foundamental and application. Prog. Bot. 60:140-166 Kim MW, Kim SJ, Kim SH, Kim BD (2001) Isolation of cDNA clones differentially accumulated in placenta of pungent pepper by suppression subtractive hybridization. Mol. Cells 11:213-219 Kim DH, Kang JG, Kim S, Kim BD (2001) Identification of coxII and atp6 region as associated to CMS in Capsicum annuum by using RFLP and long accurate PCR. J. Kor. Sci. 42:121-127 Kim DH, Kim BD (2005) Development of SCAR markers for early identification of cytoplasmic male sterility genotype in chili pepper (Capsicum annuum L.). Mol. Cells 20: 416-422 Kim DH, Kim BD (2006) The organization of mitochondria atp6 gene region in male fertile and CMS lines of pepper (Capsicum annuum L.). Curr Genet 49:59-6 Kim DS, Kim DH, Yoo JH, Kim BD (2006) Cleaved amplified polymorphic sequence and amplified fragment length polymorphism markers linked to the fertility restorer gene in chili pepper (Capsicum annuum L.). Mol. Cells 21: 135-140 Kim HK, Kang JG, Kim BD (2007) Isolation and Characterization of the cytoplasmic male sterility-associated orf456 gene of Chili pepper (Capsicum annuum L.). Plant Mol. Biol. 63:519-532 Kumar S, Singh V, Singh M, Rai S, Kumar S, Rai SK, Rai M (2007) Genetics and distribution of fertility restoration associated RAPD markers in inbreds of pepper (Capsicum annuum L.). Scientia Horticulturae 111:197-202 Lee CJ, Yoo EY, Shin JH, Hwang HS, Kim BD (2005) Non pungent Capsicum contains a deletion in the Capsaicinoid synthetase, Which allow early detection of pungency with SCAR markers. Mol. Cells 19:262-267 Lee JD, Yoon JB, Park HG (2008a) Linkage analysis between the partial restoration (pr) and the restorer-of-fertility (Rf) loci in pepper cytoplasmic male sterility. Theor. Appl. Genet. 117:383-389 Lee JD, Yoon JB, Park HG (2008b) A CAPS marker associated with the partial restoration of cytoplasmic male sterility in chili pepper (Capsicum annuum L.). Mol Breed 21: 95-104 Lefebvre V, Pflieger S, Thabuis A, Caranta C, Blattes A (2002) Toward the saturation of the pepper linkage map by alignment of three intraspecific maps including known-function gene. Genome 45:839-854 Li XQ, Jean M, Landry BS, Brown GG (1998) Restorer genes for different form of Brassica cytoplasmic male transcripts of several mitochondrial genes. Proc Natl Acad Sci USA 95:10032-10037 Liu F, Cui XQ, Horner HT, Weiner H, Schnable PS (2001) Mitochondrial aldehyde dehydrogenase activity is required for male fertility in maize. Plant Cell 13: 1063-1078 Luo XD, Dai LF, Wang SB, Wolukau JN, Jahn M, Chen JF (2006) Male gamet development and early tapetal degeneration in cytoplasmic male-sterile pepper investigated by meiotic, anatomical and ultrastructural analyses. Plant Breed 125: 395-399 Mackenzie SA, Chase CD (1990) Fertility restoration is associated with loss of a portion of the mitochondrial genome in cytoplasmic male sterile common bean. Plant Cell 2: 905-912 Martin JA, Crawford JH (1951) several type of sterility in Capsicum frutescens. Proc Am Soc Hortic Sci 57:335-338 Minamiyama Y, Kinoshita S, Inaba K, Inoue M (2005) Development of a Cleaved Amplified Polymorphic Sequence (CAPS) Marker linked to pungency in Pepper. Plant Breed 124:288-291 Min WK, Kim S, Sung SK, Kim BD, Lee S (2009) Alleleic discrimination of the Restorer-of-fertility gene and its inheritance in pepper (Capsicum annuum L.) Theor. Appl. Genet. Published online: 29 August 2009. DOI 10.1007/s00122-009-1134-y Mulyantoro, Chen SY, Wahyono A, Ku HM (2009) Modified complementation test of male sterility mutants in pepper (Capsicum annuum L.): preliminary study to convert male sterility system from GMS to CMS. Euphytica 169:353-361 Peterson PA (1958) Cytoplasmically inherited male sterility in Capsicum. Amer. Nat. 92:111-119 Pickersgill B (1977) Genetic resource and breeding of Capsicum spp. Euphytica 96:129-133 Prasad BCN, Kumar V, Gururaj HB, Parimalan R, Giridhar P, Ravishankar GA (2006) Characterization of Capsaicin Synthase and Identification of its gene (csy1) for pungency factor Capsaicin in pepper (Capsicum sp.). Proc Natl Acad Sci USA 103:13315-13320 Shifriss C (1973) Additional spontaneous male-sterile mutant in Capsicum annuum L. Euphytica 22: 527-529 Shiffris C (1997) Male sterility in pepper (Capsicum annuum L.). Euphytica 93:83-88 Shifriss C, Frankel R (1969) A new male sterility gene in Capsicum annuum L. J Amer Soc Hort Sci 94: 385-387 Shifriss C, Frankel R (1971) New sources of cytoplasmic male sterility in cultivated peppers. J Hered 64: 254-256 Shifriss C, Pilovsky M (1993) Digenic nature of male sterility in pepper (Capsicum annuum L.). Euphytica 67: 111-112 Shifriss C, Rylsky I (1972) A male sterile (ms-2) gene in 'California Wonder' pepper (Capsicum annuum L.). HortSci 7: 36. Stam P, Zeven AC (1981) The theoretical proportion of the donor genome in near isogenic lines of self fertilizers bred by backcrossing. Euphytica 30:227-238 Stewart Jr C, Kang BC, Liu K, Mazourek M, Moore SL, Yoo EY, Kim BB, Paran I, Jahn MM (2005). The Pun1 gene for pungency in pepper encodes a putative acyltransferase. The Plant J. 42:675-688 Stewart Jr C, Mazourek M, Stellari GM, Connel MO, Jahn M (2007) Genetic control of pungency in C. chinense via the Pun1 Locus. J. Exp. Bot. 58:979-991 Tang HV, Pring DR (2003) Conversion of fertility restoration of the sorghum IS1112C(A3) male sterile cytoplasm from two genes to one gene. Crop Sci 43: 1747-1753 Tanksley SD, Rick CM (1980) Isozymic gene linkage map of the tomato: application in genetics and breeding. Theor. Appl. Genet. 57:161-170 Wang ZW, Zhang YJ, Xiang CP, Mei SY, Zhou YA, Chen GP, Wang T (2008) A new fertility restorer locus linked closely to the Rfo locus for cytoplasmic male sterility in radish. Theor. Appl. Genet. 117: 313-320 Wang ZH, Zou YJ, Li XY, Zhang QY, Chen LT, Wu H, Su DH, Chen YL, Guo JX, Luo D, Long YM, Zhong Y,Liu YG (2006) Cytoplasmic male sterility of rice with Boro II cytoplasm is caused by a cytotoxic peptide and is restored by two related PPR motif gene via distinct modes of mRNA silencing. Plant Cell 18: 676-687 Wang LH, Chang BX, Lefebvre V, Huang SW, Daubeze AM, Palloix A (2004). QTL analysis of fertility restoration in cytoplasmic male sterile pepper. Theor. Appl. Genet. 109: 1058-1063 Young ND, Tanksley SD (1989) RFLP analysis of the size of chromosomal segment retained around the tm-2 locus of tomato during backcross breeding. Theor. Appl. Genet. 77:353-359 Zhang BX, Huang SW, Yang GM, Guo JZ (2000) Two RAPD markers linked to a major fertility restorer gene in pepper. Euphytica 113: 155-161
摘要: Conversion of GMS male sterility system to CMS will increase the male sterility proportion of female parent of F1 seed production from 50 to 100%. It can eliminate the cost of emasculation and ensure seed hybridity through avoidance of self pollination. In this study, segregation analysis of four male sterile mutants consisting of one CMS mutant (CA1) and three GMS mutants (GA1, GA3 and GA4) showed that each had single recessive gene inheritance. A modified complementation test was performed by replacing male sterile mutants with their maintainer line as male parent. The observation on CMS and GMS male sterility loci revealed that GA1 and GA3 had mutated in both nuclear restorer genes for CMS and GMS, while CA1 and GA4 each carried mutations in single male sterility system of nuclear restorer genes for CMS and GMS, respectively. The important finding is the broader function of a maintainer line in certain male sterility system that can be used as a maintainer or restorer line for other male sterility systems and CC1 is confirmed as the general restorer for both CMS and GMS systems. Bell pepper lacks the CMS nuclear restorer allele Rf and also contains mutations in its GMS nuclear restorer locus. Consequently the GMS maintainer and restorer lines of bell pepper are also the CMS maintainer line. This study has demonstrated the conversion of the male sterility system of the non pungent GMS bell pepper restorer line, GC3, to a CMS male sterile line using S-type cytoplasm donor (CA1) and GC3 has converted to be CMS restorer line using a Rf-allele donor (CC1). Both donors S-type cytoplasm and the Rf allele are derived from tropical pungent pepper that stable on male sterility and fertility restoration, respectively. Evaluation of S-type cytoplasm and Rf-allele introgression was conducted in BC1F1 and BC1F2 populations. Two breeding lines were selected as CMS male sterile candidates and four breeding lines were selected as CMS restorer line candidates of bell pepper. Several morphological traits of the iv selected progeny were measured and shown similar to their recurrent parent (GC3). The effectiveness of molecular markers linked to traits of interest of Stype cytoplasm, pungency and CMS nuclear restorer fertility locus were evaluated in 30 BC1F1 and 116 BC1F2 individuals. Since this study employed the stable of Rf allele and S-type cytoplasm donor from tropical pungent pepper, the developed CMS male sterile line and the restorer line candidates of bell pepper should be stable. Conversion of the male sterility system of bell pepper from GMS to CMS was performed successfully.
URI: http://hdl.handle.net/11455/36956
其他識別: U0005-2501201010292200
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2501201010292200
Appears in Collections:農藝學系

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.