Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3704
標題: 超臨界二氧化碳分離純化薑黃酮油與甲魚油生化活性物質
Separation of bioactive compounds from Curcuma longa L. and Pelodiscus sinensis by using supercritical carbon dioxide
作者: 張立勳
Chang, Li-Hsun
關鍵字: 萃取
Supercritical fluid
蒸餾
薑黃
薑黃素
薑黃酮
肝細胞
乙醇去氫酶甲魚
脂肪酸
巨噬細胞
過氧化氫酶
extraction
distillation
turmeric
curcumin
turmerone
hepatocyte
alcohol dehydrogenase (ADH)
soft shelled turtle fish
fatty acid
macrophage
catalase
出版社: 化學工程學系所
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摘要: 本研究旨在利用超臨界二氧化碳自天然原料中萃取並以管柱純化具有生物活性的物質。實驗將從兩種原料中分成三大部分進行討論。一為植物原料-薑黃,另一為動物原料-甲魚。 第一部分實驗乃利用超臨界二氧化碳在313 K至316 K與24 MPa至26 MPa條件下自新鮮薑黃植物中萃取薑黃精油。精油萃出產率為7 wt%且薑黃酮純度為71 wt%。利用NMR,薄層層析法與液相層析法進行薑黃酮的定性與定量。以低壓正相管柱層析法進行三種薑黃酮的分離純化,可成功地自超臨界二氧化碳薑黃萃出油中分離出86 %純度的ar形式及81 %純度的β+α形式之薑黃酮。萃出之薑黃精油能抑制人類肺癌細胞株的生長與大鼠巨噬細胞株吞噬作用的活性,IC50濃度各為160 μg/mL與4μg/mL。 新鮮薑黃經超臨界二氧化碳脫油後,殘餘之脫油薑黃粉內含10 wt%的薑黃素與其他具活化乙醇去氫酶(ADH)成分。利用超音波乙醇溶液在313 K下萃取脫油薑黃粉240 min,可以回收89 %的薑黃素與得到53 wt%純度的薑黃素。實驗接著將脫油薑黃粉之萃出物處理在經培養之大鼠肝細胞上並觀察細胞釋放ADH的活性。實驗結果指出,利用新鮮薑黃粉為原料與利用超臨界二氧化碳脫油薑黃粉為原料進行萃取後所獲得之兩種萃出物分別能提升16 %與35 %的ADH活性。藉由生化活性的評估,大鼠肝細胞內ADH的活性將歸因於萃出物中薑黃素的含量。 實驗最後,則利用高壓二氧化碳以底向流方式在328 K與55 bar條件下進行甲魚油囊中甲魚油的萃取與回收。回收之甲魚油酯經乙酯化後再經由對流式超臨界二氧化碳在353 K與80 bar下蒸餾油酯中之不飽和脂肪酸成分並於塔底獲得81 wt % C20-24酯肪酸乙酯。濃縮結果將利用Riccati方程式分析萃取槽內脂肪酸組成成分變化情形。濃縮後的甲魚脂肪酸乙酯能有效地促進巨噬細胞大量釋放過氧化氫酶,過氧化氫酶活性可提升至110 mole/min/mL。結果指出,魚油在生物體內的抗氧化能力可經由超臨界二氧化碳濃縮程序而被提升。
This study investigated the extraction of bioactive compounds from nature products by using supercritical carbon dioxide (SC-CO2). Experiments were performed for two materials, one is Curcuma longa L. (turmeric) and the other is Pelodiscus sinensis (soft-shelled turtle fish, SST). 7 wt% of turmeric oil yield containing 71 wt% purity of turmerones was obtained from fresh turmeric by using SC-CO2 extraction at 314 K and 25 MPa within 2.5 hr. Three major turmerones were purified from the SC-CO2 extractive turmeric oil by chromatographing in a normal phase silica gel 60 column. 86 wt% purity of ar-turmerone and 81 wt% purity of α+β-turmerone have been obtained. In vitro, this SC-CO2 extractive turmeric oil inhibited the growth of lung cancer cell (A549), showing IC50 of 160 μg/mL, and 50 % of phogocytosis activity in rat macrophage (Raw 264.7) was also inhibited by 4μg/mL of this extracted oil. Following SC-CO2 deoiled process of turmeric material, the residue of SC-CO2 extracted turmeric contains 10 wt% of curcumins and other active compounds of activating alcohol dehydrogenase (ADH). These bioavailable compounds were obtained from this SC-CO2 extracted turmeric by using an ethanol solution ultrasonicated at 313 K for 240 min. 89 % of curcumins was successfully recovered and 53 wt% purity of curcumins was obtained. The ADH activity in rat hepatocytes treated with these extracts of deoiled turmerics has been assayed in vitro. Our experimental results indicated that the extracts of fresh turmeric and SC-CO2 deoiled turmeric increased the ADH enhancement factor by 16 % and 35 %, respectively. This bioavailability investigation clearly evidenced that the enhancing ADH activity in rat hepatocytes was mainly attributed to the content of curcummins in the extract. Finally, this work studied the recovery of omega-3 fatty acids from SST oil bags. 53 wt% crude oil yield was obtained by using pressurized carbon dioxide with top-down fluid flow at 55 bar and 328 K. 81 wt% of C20-24 fatty acid was then enriched using a counter-current SC-CO2 extractive distillation from an esterified oil mixture at 353 K and 250 bar. The column-height versus concentration of C20-24 was examined using the Riccati equation. This refined oil promoted the catalase release of rat macrophage, showing 110 mole/min/mL of catalase activity. The results indicated that the anti-oxidant activity of SST oil was enhanced after SC-CO2 concentration process.
URI: http://hdl.handle.net/11455/3704
其他識別: U0005-2508200813493300
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