Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/37045
DC FieldValueLanguage
dc.contributor.advisor王慶裕zh_TW
dc.contributor.advisorWei-Yu Chouen_US
dc.contributor.advisor周煒裕zh_TW
dc.contributor.author林訓仕zh_TW
dc.contributor.authorLin, Hsun-Shihen_US
dc.date2005zh_TW
dc.date.accessioned2014-06-06T07:58:33Z-
dc.date.available2014-06-06T07:58:33Z-
dc.identifier.urihttp://hdl.handle.net/11455/37045-
dc.description.abstract本試驗主要利用五個水稻基因型為材料,在培養基中添加不同濃度固殺草,觀察是否能誘導微芽大量生成,結果顯示在培養第一階段添加2.5×10-7 M固殺草並繼代至第二階段2.5×10-6 M固殺草培養基中,對水稻R11-2及R11-3能有最佳之微芽誘導效果。將上述兩階段誘導衍生之植株移植溫室,發現在葉綠素及銨累積反應上,對於固殺草具有耐感差異。進一步利用14C-glufosinate處理植株,經TLC分析及14C同位素放射活性測定,推測微芽衍生植株之間耐感原因為其對固殺草代謝能力上之差異所致。 為了探討經固殺草誘導衍生之耐感水稻是否對其他除草劑具有多重耐性,乃將上述移植溫室篩選出之耐感水稻,同時測定其對本達隆之耐感反應,根據其抑制PS II activity,發現衍生植株在本達隆的耐感性表現上與固殺草耐感性反應相似,尤其FSK及R11-2基因型。 為了增大水稻對於除草劑耐感差異之程度,固殺草誘導水稻微芽產生時,配合疊氮化鈉處理,試驗發現經誘變後之植株,在固殺草耐感反應上具有顯著之差異。zh_TW
dc.description.abstractIn order to promote microshoot production of rice, different concentrations of glufosinate were applied in tissue culture of 5 genotypes. Experimental results showed that the best effect of microshoot induction was obtained from the treatment combination of 2.510-7 M glufosinate at stage I and 2.510-6 M glufosinate at stage II for rice line R11-2 and R11-3. Microshoot-derived plants produced from this method expressed differential sensitivities to glufosinate in greenhouse experiment, based on the response of chlorophyll reduction and ammonium accumulation. Both TLC and 14C-glufosinate radioactivity assay showed that glufosinate metabolism was increased in tolerant rice. In addition, multiple tolerance to bentazon, an inhibitor of PS II activity, was also found, especially for the genotype FSK and R11-2. The result showed in this experiment that using sodium azide to enlarge the variation of rice resistance to glufosinate was effective.en_US
dc.description.tableofcontents目錄 頁次 目錄………………………………………………………………… i 表目錄…………………………………………………………… ii 圖目錄…………………………………………………………… v 壹、緒言………………………………………………………………. 1 貳、前人研究……………………………………………………… 4 參、材料與方法…………………………………………………… 13 肆、結果與討論…………………………………………………… 23 一、 Glufosinate對水稻不同基因型(genotype)間微芽誘導效果 之比較…………………………………………………………23 二、不同基因型水稻經glufosinate誘導對微芽對除草劑耐性 變異……………………………………………………………..48 三、NaN3誘致微芽產生除草劑耐性變異之效果…………………66 四、微芽衍生植株除草劑耐性變異之可能原因…….……………..70 伍、中文摘要……………………………………………………… 75 陸、英文摘要……………………………………………………… 76 柒、參考文獻……………………………………………………… 77 表目錄 頁次 表 1.台農67切離芽移植至含不同濃度固殺草培養基後之生長反應………..24 Table 1. Effect of glufosinate on the growth of buds directly excised from 3-day old germinating seeds of rice, cv. TNG 67…………………………24 表 2.固殺草誘導台農67微芽產生之效果-第二階段………………………….26 Table 2. Effect of glufosinate applied at two incubation stages on the subsequent production of microshoots derived from excised buds of 3-day germinating seeds of rice, cv. TNG 67…………………………..26 表 3. 富士光切離芽移植至含不同濃度固殺草培養基後之生長反應………..28 Table 3. Effect of glufosinate on the growth of buds directly excised from 3-day old germinating seeds of rice, cv. FSK……………………………..28 表 4. 固殺草誘導富士光微芽產生之效果-第二階段…………………………30 Table 4. Effect of glufosinate applied at two incubation stages on the subsequent production of microshoots derived from excised buds of 3-day germinating seeds of rice, cv. FSK……………………………...30 表 5. 越光切離芽移植至含不同濃度固殺草培養基後之生長反應………….33 Table 5. Effect of glufosinate on the growth of buds directly excised from 3-day old germinating seeds of rice, cv. Koshihikari…………………….33 表 6. 固殺草誘導越光微芽產生之效果-第二階段…………………………...34 Table 6. Effect of glufosinate applied at two incubation stages on the subsequent production of microshoots derived from excised buds of 3-day germinating seeds of rice, cv. Koshihikari……………………..34 表 7. 水稻突變體R11-2切離芽移植至含不同濃度固殺草培養基後 之生長反應…………………………………………………………….36 Table 7. Effect of glufosinate on the growth of buds directly excised from 3-day old germinating seeds of rice, R11-2………………………….…36 表 8. 固殺草誘導水稻突變體R11-2微芽產生之效果-第二階段……………38 Table 8. Effect of glufosinate applied at two incubation stages on the subsequent production of microshoots derived from excised buds of 3-day germinating seeds of rice, line R11-2……………………………38 表 9. 水稻突變體R11-3切離芽移植至含不同濃度固殺草培養基後………..40 之生長反應 Table 9. Effect of glufosinate on the growth of buds directly excised from 3-day old germinating seeds of rice, R11-3………………………………..40 表 10. 固殺草誘導水稻突變體R11-3微芽產生之效果-第二階段…………..42 Table 8. Effect of glufosinate applied at two incubation stages on the subsequent production of microshoots derived from excised buds of 3-day germinating seeds of rice, line R11-3…………………………..42 表 11. 2.5×10-6 M固殺草對5個不同基因型水稻之生長影響………………45 Table 11.Comparison of the effect of glufosinate (2.5×10-6 M)in culture medium on the growth inhibition of buds excised from 3-day-old germinating seeds of rice among five rice plants………………………..45 表 12. 固殺草誘導5個不同基因型水稻微芽產生之效果………………….46 Table 12. Effect of glufosinate applied at two incubation stages on the subsequent production of microshoots derived from excised buds of 3-day germinating seeds of rice………………………………...46 表 13. 台農67葉片片段處理0.26 mM固殺草後,葉綠素及銨累積情形..50 Table 13. Chlorophyll reduction and ammonium accumulation in leaf segment of rice , cv. TNG 67, 24 h after 0.26 mM glufosinate treatment……….50 表 14. 富士光葉片片段處理0.26 mM固殺草後,葉綠素及銨累積情形…53 Table 14. Chlorophyll reduction and ammonium accumulation in leaf segment of rice , cv. FSK, 24 h after 0.26 mM glufosinate treatment……………53 表 15. 越光葉片片段處理0.26 mM固殺草後,葉綠素及銨累積情形……56 Table 15. Chlorophyll reduction and ammonium accumulation in leaf segment of rice , cv. koshihikari, 24 h after 0.26 mM glufosinate treatment…….56 表 16. 水稻突變體R11-2葉片片段處理0.26 mM固殺草後,葉綠素及 銨累積情形……………………………………………………………….59 Table 16. Chlorophyll reduction and ammonium accumulation in leaf segment of rice , line R11-2, 24 h after 0.26 mM glufosinate treatment……………...59 表 17. 水稻突變體R11-3葉片片段處理0.26 mM固殺草後,葉綠素及 銨累積情形……………………………………………………………….62 Table 17. Chlorophyll reduction and ammonium accumulation in leaf segment of rice , line R11-3, 24 h after 0.26 mM glufosinate treatment……………...62 表 18. 經疊氮化鈉處理之水稻突變體R11-2,其微芽誘導效果……………….68 Table 18. Effect of sodium azide on the microshoot production induced by glufosinate at two incubation stages of buds excised from 3-d germinating seeds of rice line R11-2……………………………………………………….68 表19.處理14C固殺草24小時後之代謝情形…………………………………….73 Table 19. Metabolism of 14C-glufosinate in 24 h in leaves of rice plants at tillering stage………………………………………………………………...73 圖目錄 頁次 圖1. 台農67第一階段第14天對固殺草之生長反應………………………….25 Figure 1. Seedling growth of rice, cv. TNG 67, 14 days after culturing buds in medium containing 0, 2.5×10-9, 2.5×10-8, 2.5×10-7 and 2.5×10-6 M glufosinate (from left to right), respectively, at stage I…....25 圖2. 富士光第一階段第14天對固殺草之生長反應……………………………29 Figure 2. Seedling growth of rice, cv. FSK, 14 days after culturing buds in medium containing 0, 2.5×10-9, 2.5×10-8, 2.5×10-7 and 2.5×10-6 M glufosinate (from left to right), respectively, at stage I…….29 圖3. 越光第一階段第14天對固殺草之生長反應………………………………32 Figure 3. Seedling growth of rice, cv. Koshihikari, 14 days after culturing buds in medium containing 0, 2.5×10-9, 2.5×10-8, 2.5×10-7 and 2.5×10-6 M glufosinate (from left to right), respectively, at stage I…….32 圖4. 水稻突變體R11-2第一階段第14天對固殺草之生長反應……………… 37 Figure 4. Seedling growth of rice, line R11-2, 14 days after culturing buds in medium containing 0, 2.5×10-9, 2.5×10-8, 2.5×10-7 and 2.5×10-6 M glufosinate (from left to right), respectively, at stage I…….37 圖5. 水稻突變體R11-3第一階段第14天對固殺草之生長反應……………….41 Figure 5. Seedling growth of rice, line R11-3, 14 days after culturing buds in medium containing 0, 2.5×10-9, 2.5×10-8, 2.5×10-7 and 2.5×10-6 M glufosinate (from left to right), respectively, at stage I…….41 圖6. 5個基因型水稻之微芽誘導產生……………………………………………47 Figure 6. Microshoot production of rice, (A) TNG 67, (B) FSK, (C) Koshihikari, (D) R11-2, and (E) R11-3, respectively, 30 days after 2.5×10-6 M glufosinate treatment at stage II. Five sets of rice plants were pretreated with 2.5×10-7 M glufosinate at stage I…………………………………….47 圖7. 台農67噴施1.5 mM固殺草後,7、14及21天之傷害指數頻度分佈...49 Figure 7. Frequency distribution of injury index of rice seedlings caused by 1.5 mM glufosinate, rice seedlings were derived from microshoots of excised buds of rice cv. TNG 67……………………………………………49 圖8. 富士光噴施1.5 mM固殺草後,7、14及21天之傷害指數頻度分佈…52 Figure 8. Frequency distribution of injury index of rice seedlings caused by 1.5 mM glufosinate, rice seedlings were derived from microshoots of excised buds of rice cv. FSK………………………………………………52 圖9. 越光噴施1.5 mM固殺草後,7、14及21天之傷害指數頻度分佈……55 Figure 9. Frequency distribution of injury index of rice seedlings caused by 1.5 mM glufosinate, rice seedlings were derived from microshoots of excised buds of rice cv. Koshihikari……………………………………….55 圖10. 水稻突變體R11-2噴施1.5 mM固殺草後,7、14及21天之傷害 指數頻度分佈……………………………………………………………58 Figure 10. Frequency distribution of injury index of rice seedlings caused by 1.5 mM glufosinate, rice seedlings were derived from microshoots of excised buds of rice line R11-2……………………………………………58 圖11. 水稻突變體R11-3噴施1.5 mM固殺草後,7、14及21天之傷害 指數頻度分佈……………………………………………………………61 Figure 11. Frequency distribution of injury index of rice seedlings caused by 1.5 mM glufosinate, rice seedlings were derived from microshoots of excised buds of rice line R11-3……………………………………………61 圖12. 微芽衍生之耐感植株在固殺草銨累積及本達隆光合作用系統II 之抑制情形…………………………………………………………….65 Figure 12. Multiple tolerance of rice plants to both glufosinate and bentazon. Bentazon at 0.98 mM and glufosinate at 1.5 mM were applied to leaf segments of rice plants at tillering stage, respectively, A: TNG 67、 B: FSK、C: R11-2 and D: R11-3………………………………………...65 圖13. 經疊氮化鈉誘變之微芽所衍生之水稻葉片片段處理0.26 mM固殺草 一天後其葉綠素及銨累積反應………………………………………69 Figure 13. Chlorophyll reduction and ammonium accumulation in leaf segments of microshoot-derived plant of rice, 24 h after 0.26 mM glufosinate treatment……………………………………………………..69 圖14. 利用TLC分離技術及自動顯影術偵測水稻耐性FSK(編號1.2.3) 及感性FSK(編號4.5.6)及對照組台農67(編號7.8.9),經固殺草 處理後24小時其植體內固殺草(Glu, Rf:0.20)、未知代謝物(UN, Rf:0.34)及其代謝物(MPP, Rf:0.55)的變化………………………………72 Fig. 14. Autoradiography, coupled with TLC separation, of the 14C-glufosinate(Glu ; Rf:0.20)、unknown metabolite (UN ; Rf:0.34) and its metabolite(MPP, Rf:0.55). Samples were extracted from 14C-glufosinate treated rice induced the tolerant FSK (1-3)、sensitive FSK (4-6) and control line TNG 67 (7-9), respectively, 24 h after treatment…………………………………………….72zh_TW
dc.language.isoen_USzh_TW
dc.publisher農藝學系zh_TW
dc.subjectmicroshooten_US
dc.subject微芽zh_TW
dc.subjectglufosinateen_US
dc.subject固殺草zh_TW
dc.title水稻微芽之誘導分化及其對除草劑之耐性zh_TW
dc.titleDifferentiation induction of rice microshoots and its tolerance to herbicideen_US
dc.typeThesis and Dissertationzh_TW
Appears in Collections:農藝學系
文件中的檔案:

取得全文請前往華藝線上圖書館

Show simple item record
 
TAIR Related Article
 
Citations:


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.