Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/37265
標題: 利用單子葉簡單重複序列分子標誌分析台灣原生秋海棠的遺傳歧異度
Application of transferable monocot SSR markers for analyzing genetic diversity of Taiwan native Begonia
作者: 羅子絹
Lo, Tzu-Chuan
關鍵字: 秋海棠
Begonia
簡單重複序列分子標誌
轉移率
種源多樣性
simple sequence repeat (SSR) markers
transferability
genetic diversity
出版社: 農藝學系所
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摘要: 秋海棠屬(Begonia L.) 是維管束植物中第六大屬,有超過1500種原生種遍布於熱帶與亞熱帶地區。台灣共有18種已被記錄的原生秋海棠,其中的14種分別屬於3個組(section):秋海棠組(sect. Diplocinium)、扁果組(sect. Platycentrum) 以及無翅組(sect. Sphenanthera),另外4種為天然雜交種。台灣為世界已知秋海棠天然雜交種和物種特有率最多的地區,也是秋海棠染色體型態最複雜的亞洲地區,此外,台灣原生秋海棠具有觀賞和藥用之價值,因此物種的鑑別和多樣性研究對原生秋海棠的應用具有重要性。本試驗利用120個來自單子葉作物的SSR分子標誌進行台灣原生秋海棠的遺傳多樣性分析,以測試單子葉SSR分子標誌應用於遠緣植物的可行性,並且藉此了解台灣原生秋海棠的親緣關係。結果顯示平均60.6%的單子葉SSR分子標誌可以增幅秋海棠各原生種的基因組,而單子葉SSR分子標誌的轉移率會受到SSR重複序列的重複類型、長度、以及分布於基因區與否所影響,其中以SSR分子標誌在基因組的分布對轉移率影響最為明顯,位於基因區的SSR分子標誌增幅秋海棠基因組的能力和多型性程度顯著的高於位於非基因區的SSR分子標誌。進一步分別以基因區分子標誌、非基因區分子標誌、以及綜合以上兩者的增幅結果進行親緣性分析,可將台灣原生秋海棠成功地分成三群,而蘭嶼秋海棠(B. fenicis)、岩生秋海棠(B. ravenii)、以及裂葉秋海棠(B. palmata)與此三群原生秋海棠的親緣關係較遠。利用分子標誌分類結果與型態分類不一致,但與染色體數目相關,推測可能是由於台灣原生秋海棠發生種間雜交和平行演化的結果。本篇論文證明單子葉SSR分子標誌可跨物種轉移的可行性,未來將可提供序列資訊缺乏之植物作為種源多樣性與親緣關係研究的工具。
Begonia is the sixth-largest genus of vascular plants in the world. There are fourteen native species and four endemic natural hybrids in Taiwan in which the highest density of natural hybrids and species containing the most complex chromosome numbers in Asia have been reported. Besides, the native Begonia species in Taiwan show great potential as ornamental and medical plants. However, very few molecular tools have been conducted in Begonia species identification and diversity investigation. This study had employed 120 SSR markers designed from monocot genomes to test transferability on Begonia species in Taiwan. An average of 60.6% transferability and multi-unique loci with 100% polymorphism were obtained. In addition, the degree of transferability was influenced by SSR motif types, length and distribution in coding or noncoding regions especially. Simple and imperfect type showed higher transferability. Meanwhile, transferability values of tetra- and dinucleotide repeats were higher than those of tri-nucleotide. Markers derived from coding region revealed higher polymorphism and transferability than those of noncoding region markers. The dendrograms penetrated by data from coding region markers was more similar to total region markers than those of noncoding region markers in diversity analysis. Three groups of dendrograms based on coding, noncoding and total markers were in accordance with previous studies of phylogeny of Begonia species in Taiwan. It demonstrated that SSR markers derived from monocot genomes can be applied in genetic diversity and phylogenetic analysis of distantly related dicotyledons.
URI: http://hdl.handle.net/11455/37265
其他識別: U0005-1207201311502900
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-1207201311502900
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