請用此 Handle URI 來引用此文件：
Isolation and Analysis of B-chromosome Specific cDNA-AFLP Markers in Maize
Chang, Stephen Kuo-Hu
B-chromosome Specific cDNA-AFLP Marker
|引用:||參考文獻 Alfenito, M.R. and J.A. Birchler. 1993. Molecular characterization of a maize B chromosome centric sequence. Genetics 135:589-597. Auger, D. and J. Birchler. 2002. Maize tertiary trisomic stocks derived from B-A translocations. J. Hered. 93:42-47. Beckett, J.B. 1978. B-A translocations in maize I. Use in locating genes by chromosome arms. J. Hered. 69:27-36. Birchler, J.A. 1979. A study of enzyme activities in a dosage series of the long arm of chromosome one in maize. Genetics 92:1211-1229. Carchilan, M., K. Kumke, S. Mikolajewski, and A. Houben. 2009. Rye B chromosomes are weakly transcribed and might alter the transcriptional activity of A chromosome sequences. Chromosoma 118:607-616. Carlson, W.R. 1969. Factors affecting preferential fertilization in maize. Genetics 62:543-554. Carlson, W.R. and R.R. Roseman. 1992. A new property of the maize B chromosome. Genetics 131:211-223. Cheng, Y.M. 2010. Evolution of the heterochromatic regions on maize B long arm based on the sequence structure of CL-repeat variants. Chromosome Res. 18:605-619. Cheng, Y.M. and B.Y. Lin. 2003. Cloning and characterization of maize B chromosome sequences derived from microdissection. Genetics 164:299-310. Cheng, Y.M. and B.Y. Lin. 2004. Molecular organization of large fragments in the maize B chromosome: Indication of a novel repeat. Genetics 166:1947-1961. Gonzalez-Sanchez, M., E. Gonzalez-Gonzalez, F. Molina, A. Chiavarino, M. Rosato, and M.J. Puertas. 2003. One gene determines maize B chromosome accumulation by preferential fertilisation; another gene (s) determines their meiotic loss. Heredity 90:122-129. Gotoh, K. 1924. Uber die chromosomenzahl von Secale cereale L. Bot. Mag. Tokyo 38:135-152. Jones, N. and A. Houben. 2003. B chromosomes in plants: Escapees from the A chromosome genome? Trends Plant Sci. 8:417-423. Jones, R.N., W. Viegas, and A. Houben. 2008. A century of B chromosomes in plants: So what? Ann. Bot. 101:767-775. Kato, A., J.C. Lamb, and J.A. Birchler. 2004. Chromosome painting using repetitive DNA sequences as probes for somatic chromosome identification in maize. Proc. Natl. Acad. Sci. USA 101:13554-13559. Kuwada, Y. 1915. Ueber die chromosomenzahl von Zea mays L. Bot. Mag. Tokyo 29:83-89. Lamb, J.C., A. Kato, and J.A. Birchler. 2005. Sequences associated with A chromosome centromeres are present throughout the maize B chromosome. Chromosoma 113:337-349. Lamb, J.C., N.C. Riddle, Y.M. Cheng, J. Theuri, and J.A. Birchler. 2007. Localization and transcription of a retrotransposon-derived element on the maize B chromosome. Chromosome Res. 15:383-398. Lin, B.Y. 1972. Synthesis of a set of B-A translocations involving a given segment of chromosome 10. Maize Genet. Coop. News Lett. 46:193-194. Lin, B.Y. 1974. TB-10 breakpoints and marker genes on the long arm of chromosome 10. Maize Genet. Coop. News Lett. 48:182-184. Lin, B.Y. 1979. Two new B-10 translocations involved in the control of nondisjunction of the B chromosome in maize. Genetics 92:931-945. Lin, B.Y. and H.P. Chou. 1997. Physical mapping of four RAPDs in the B chromosome of maize. Theor. Appli. Genet. 94:534-538. Lo, K.L., Y.P. Lin, L.J. Chen, and B.Y. Lin. 2009a. Isolation and characterization of new maize B sequences from a microdissected library. Plant Mol. Biol. Rep. 27:350-354. Lo, K.L., S.F. Peng, L.J. Chen, and B.Y. Lin. 2009b. Tandem organization of StarkB element (22.8 kb) in the maize B chromosome. Mol. Genet. Genomics 282:131-139. Longley, A.E. 1927. Supernumerary chromosomes in Zea mays. J. Agric. Res. 35:769-784. Nagaki, K., J. Song, R.M. Stupar, A.S. Parokonny, Q. Yuan, S. Ouyang, J. Liu, J. Hsiao, K.M. Jones, and R.K. Dawe. 2003. Molecular and cytological analyses of large tracks of centromeric DNA reveal the structure and evolutionary dynamics of maize centromeres. Genetics 163:759-770. Peng, S.F., Y.P. Lin, and B.Y. Lin. 2005. Characterization of AFLP sequences from regions of maize B chromosome defined by 12 B-10L translocations. Genetics 169:375-388. Randolph, L.F. 1928. Types of supernumerary chromosomes in maize. Anat. Rec. 41:102. Roman, H. 1948. Directed fertilization in maize. Proc. Natl. Acad. Sci. USA 34:36-42. Roman, H. and A.J. Ullstrup. 1951. The use of A-B translocations to locate genes in maize. Agron. J. 43:450-454. Shan, X., T. Blake, and L. Talbert. 1999. Conversion of AFLP markers to sequence-specific PCR markers in barley and wheat. Theor. Appli. Genet. 98:1072-1078. Stark, E.A., I. Connerton, S.T. Bennett, S.R. Barnes, J.S. Parker, and J.W. Forster. 1996. Molecular analysis of the structure of the maize B-chromosome. Chromosome Res. 4:15-23. Vos, P., R. Hogers, M. Bleeker, M. Reijans, T. Lee, M. Hornes, A. Friters, J. Pot, J. Paleman, and M. Kuiper. 1995. AFLP: A new technique for DNA fingerprinting. Nucleic Acids Res. 23:4407-4414. Ward, E.J. 1973. Nondisjunction: Localization of the controlling site in the maize B chromosome. Genetics 73:387-391. Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski, and S.V. Tingey. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18:6531-6535. Wilson, E.B. 1907. The supernumerary chromosomes of hemiptera. Science 26:870-871.|
Abstract In the present research, maize inbred lines (L289 and W23) with and without B chromosomes, and the TB-10L38 translocation inbred line were used to isolate B-chromosome specific cDNA-AFLP markers by using cDNA-AFLP analysis. A total of 44 B-chromosome specific cDNA-AFLP markers were isolated from L289 inbred lines with and without B chromosomes, and 39 of which were cloned successfully. By using W23 inbred lines with and without B chromosomes and the TB-10L38 translocation, 8 B-chromosome specific cDNA-AFLP markers were isolated, and then 6 of which were cloned and sequenced. Sequence analysis showed that 4 out of 45 cDNA-AFLP markers had homology with B-chromosome related sequence, 28 markers had homology with sequence of different gene, mRNA, or BAC clone sequences in maize, and 13 markers showed no homology with any published sequence. Southern hybridization analysis indicated that 10 markers showed B-specificity or dosage effect signals, 20 markers had identical signals in both genomic DNA with and without B chromosomes and the remaining 15 markers showed no signal. Therefore, sequences of 2 markers with B-chromosome related sequence, 3 markers with B-specificity or dosage effect signals, and 3 markers randomly chosen were used to design primers for RT-PCR and SCAR-PCR analysis. Results of RT-PCR showed that 4 markers could be amplified from RNA of L289 with and without B chromosomes indicated that these markers had transcriptional activity. SCAR-PCR results showed that these 4 markers could be amplified from DNAs of L289 with and without B chromosomes, indicating these 4 markers were existed in genomic DNA and had the ability for expression.
在 DSpace 系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。