Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3729
標題: 製備多層壁奈米碳管奈米複合材料做為乙醇和兒茶素感測器之探討
Preparation of multiwalled carbon nanotube nanocomposites for ethanol and catechin sensors
作者: 李振安
Lee, Chen-An
關鍵字: carbon nanotube
乙醇去氫酶乙醇
alcohol dehydrogenase
ethanol
catechin
兒茶素
出版社: 化學工程學系所
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摘要: 摘要 本研究成功的利用奈米生物複合薄膜製備出安培法生物感測器偵測乙醇,奈米生物複合薄膜由多層壁奈米碳管(multiwalled carbon nanotubes, MWNTs)、幾丁聚醣(chitosan, CHIT)、酒精去氫酶(alcohol dehydrogenase, ADH)組成並塗佈在玻璃碳電極(glassy carbon electrode, GCE)上。此MWNTs-CHIT-ADH奈米生物複合薄膜之分散均勻與否在本研究中以場發射電子顯微鏡(field emission scanning electron microscopy,FESEM)觀察並作探討。在此研究中使用循環伏安法和安培法作為MWNTs-CHIT-ADH 奈米生物複合薄膜偵測乙醇之催化活性研究。在此乙醇感測研究中針對一系列之重要參數做最適化研究,這些重要參數包括了工作電位、溶液之pH值、輔酶(NAD+)濃度、酵素負載量等。此最適化生物感測器經整理後可得到ADH最適量為10 mg ml-1,靈敏度0.1646 A M-1cm-2,Michaelis-Menten 常數0.38 mM,偵測極限0.52 µM。最後為了能將此乙醇生物感測器應用在市售酒精性飲料,因此針對台灣啤酒、紅葡萄酒及高粱酒等作檢測,發現與商用標示相當接近,表示此酒精生物感測器可精確得到市 售酒精濃度。 在兒茶素感測研究中,採用化學感測器做測量,且以方波伏安法進行測試,發現以MWNTs-ACS修飾GCE之工作電極在對兒茶素偵測時可以得到很高的靈敏度。可得效能參數分別為靈敏度245.7778 A M-1 cm-2,偵測極限0.141µM。接著針對市售茶類飲料所含兒茶素含量做偵測,發現偵測值在商標所標示之物差範圍內,表示此化學感測 器可準確得知兒茶素含量。
Abstract This study includes the ethanol biosensors and the catechin chemical sensors. The first part is the determination of ethanol by using multiwalled carbon nanotubes (MWNTs), a chitosan (CHIT) binder, and an alcohol dehydrogenase (ADH) function to form a nanbiocomposite film. The MWNTs-CHIT-ADH nanobiocomposite film is cast on a glassy carbon electrode. Homogeneity of the resulting nanobiocomposite film was investigated by field emission scanning electron microscopy(FE-SEM). Electrocatalytic activity of the MWNTs-CHIT-ADH nanobiocomposite was determined using cyclic voltammetry and amperometry. Optimum conditions of the biosensor were experimentally determined through varying several important parameters. These included working potential, solution pH value, NAD+ concentration, and enzyme loading. The optimized biosensor shows a sensitivity of 0.1646 A M-1 cm-2, an apparent Michaelis–Menten constant of 0.38 mM, and a detection limit of 0.52 μM. The applicability of the proposed biosensor based on MWNTs-CHIT-ADH nanobiocomposite was tested by the detection of ethanol in beer, red wine, and spirit. The second part of determination of catechin has been performed at this study. The MWNTs are dispersed into ACS aqueous solution by ultrasonic and adsorption on a glassy carbon electrode. The MWNTs-ACS nanocomposite was characterized by SEM. The sensitivity of the catechin sensor is much higher by using the MWNTs-ACS modified glassy carbon electrode. The chemical sensor of catechin displays a sensitivity of 246. 7778 A M-1 cm-2 and a detection limit of 0.141 µM 。
URI: http://hdl.handle.net/11455/3729
其他識別: U0005-1007200914040900
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-1007200914040900
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