Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3764
標題: 人類內皮細胞在不同濃度奈米金/銀-聚胺酯複合材料上之行為評估
Behavior of human endothelial cells on the nanocomposites of poly(ester)urethane and gold/silver nanoparticles of different concentrations.
作者: 何統作
Ho, Tung-Tso
關鍵字: 2-D
二維電泳
polyurethane
nanocomposite
nano Au
nano Ag
cell behavior
endothelial cell
聚胺酯
複合材料
奈米金
奈米銀
細胞行為
內皮細胞
出版社: 化學工程學系所
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摘要: 本研究以二維電泳以及基因表現等方式,試圖找出內皮細胞受到不同濃度之奈米金-聚胺酯以及奈米銀-聚胺酯複合材料影響,調控開啟的訊息傳遞路徑,以找出不同的材料對於細胞之間的不同影響途徑。實驗結果發現,含43.5 ppm 奈米金的奈米金-聚胺酯,以及含30 ppm 奈米銀的奈米銀-聚胺酯,皆能明顯促進細胞生長以及Lamellipodia的形成,然而奈米金-聚胺酯似乎是透過Akt影響VCP進而促進eNOS活化,導致此項結果;而奈米銀-聚胺酯卻並非經由此種途徑來調控細胞。
This study is focused on how materials affect cell behavior and what signal transduction pathways are turned on when endothelial cells are grown on poly(ester)urethane (PSU)-Au/Ag nanocomposites. The activation of proteins and genes were analyzed by two-dimensional gel electrophoresis and reverse transcription PCR (RT-PCR). It was found that PSU-Au 43.5 ppm and PSU-Ag 30 ppm enhanced the proliferation and lamellipodia formation of endothelial cells. PSU-Au appeared to influence cell behavior by activation of valosin-containing protein (VCP) and the downstream molecule endothelial nitric oxide synthase (eNOS). On the other hand, PSU-Ag seemed to influence cell behavior via a different pathway.
URI: http://hdl.handle.net/11455/3764
其他識別: U0005-2008200910511400
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2008200910511400
Appears in Collections:化學工程學系所

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