請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/37698
標題: Molecular mechanisms responsible for microglia-derived protection of Sprague-Dawley rat brain cells during in vitro ischemia
作者: Lu, Y.Z.
葛其梅
Lin, C.H.
Cheng, F.C.
Hsueh, C.M.
關鍵字: in vitro ishchemia
microglia
astrocyte
neuron
GDNF
TGB-beta 1
middle cerebral-artery
transforming growth-factor-beta-1
gdnf
expression
beta-s
transient
occlusion
tgf-beta-1
survival
injury
degeneration
期刊/報告no:: Neuroscience Letters, Volume 373, Issue 2, Page(s) 159-164.
摘要: Microglia-derived protection of brain cells (microglia, astrocytes, and neurons) during in vitro ischemic stress (deprivation of glucose, oxygen, and serum) was determined. Trypan blue exclusion assay, immunoblocking assay, Western blot analysis. and ELISA assay were used to determine the molecular mechanisms responsible for the microglia-derived protection. Results demonstrated that supermatants from the ischemic microglia protected all three cell-types from ischemia-induced damage by releasing the transforming growth factor-beta1 (TGF-beta1) and glial cell line-derived neurotrophic factor (GDNF). The protection of microglia was TGF-beta1 related, whereas astrocytes protection was GDNF-dependent. The protection of neurons was TGF-beta1 and GDNF independent. and the molecular nature responsible for their protection remains to be determined. These results indicate contribution from the surrounding cells and the types of receptors expressed on different brain cells probably also play an important role in determining their fate against ischemia. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
URI: http://hdl.handle.net/11455/37698
ISSN: 0304-3940
文章連結: http://dx.doi.org/10.1016/j.neulet.2004.10.004
顯示於類別:生命科學系所

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