Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/37717
標題: The biotechnological potential of the extreme halophilic archaea Haloterrigena sp H13 in xenobiotic metabolism using a comparative genomics approach
作者: Ding, J.Y.
賴美津
Lai, M.C.
關鍵字: comparative genomics
extremophiles
halophilic archaea
xenobiotic
degradation
halobacterium species nrc-1
organic-solvent tolerance
methanosarcina-thermophila
beta-hexachlorocyclohexane
l-2-haloacid
dehalogenase
reductive dechlorination
degradation
sequence
pathway
strain
期刊/報告no:: Environmental Technology, Volume 31, Issue 8-9, Page(s) 905-914.
摘要: Extreme halophilic archaea are thriving and dominant populations within hypersaline environments. Because of the extreme properties of the enzymes of halophilic archaea and similar metabolic abilities to their bacterial counterparts, our interests focus on their potential biotechnological applications. In this study, the partial genome of a newly isolated extreme halophilic archaeon, Haloterrigena sp. H13, was investigated. The genome size was estimated to be about 3.9 MB, and a genomic shotgun library was constructed. A total of 1479 clones from the library were sequenced once, and 1186 contigs were obtained. From these contigs, 580 open reading frames (ORFs) were identified, and 394 ORFs were annotated. From the partial genome of strain H13, we identified genes that may be involved in 1,2-dichloroethane degradation, naphthalene/anthracene degradation, -hexachlorocyclohexane degradation, 1-/2-methylnaphthalene degradation and benzoate degradation via CoA ligation. Among the identified ORFs, gene homologs of (S)-2-haloacid dehalogenase (EC 3.8.1.2) and salicylate hydroxylase (EC 1.14.13.1), which might be involved in the degradation of dichloroethane, -hexachlorocyclohexane and naphthalene, were found in the partial genome sequence of strain H13. According to the current genome annotation of peripheral metabolic pathways and the putative xenobiotic-degrading enzymes, the potential of extreme haloarchaea in bioremediation applications is proposed.
URI: http://hdl.handle.net/11455/37717
ISSN: 0959-3330
文章連結: http://dx.doi.org/10.1080/09593331003734210
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