Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/37750
標題: Differential responses in gills of euryhaline tilapia, Oreochromis mossambicus, to various hyperosmotic shocks
作者: Wang, P.J.
李宗翰
Lin, C.H.
Hwang, L.Y.
Huang, C.L.
Lee, T.H.
Hwang, P.P.
關鍵字: Euryhaline teleost
Gill
Mitochondrion-rich cells
Na plus /K plus
-ATPase
Osmolality
Tilapia
mitochondria-rich cells
na+-k+-atpase
killifish fundulus-heteroclitus
branchial chloride cells
atlantic salmon smolts
bass morone-saxatilis
time-course changes
rainbow-trout gill
yolk-sac membrane
fresh-water
期刊/報告no:: Comparative Biochemistry and Physiology a-Molecular & Integrative Physiology, Volume 152, Issue 4, Page(s) 544-551.
摘要: Euryhaline tilapia (Oreochromis mossambicus) survived in brackish water (BW; 20 parts per thousand) but died in seawater (SW; 35 parts per thousand) within 6 h when transferred directly from fresh water (FW). The purpose of this study was to clarify responses in gills of FW tilapia to various hyperosmotic shocks induced by BW or SW. In FW-acclimated tilapia, scanning electron micrographs of gills revealed three subtypes of MR cell apical surfaces: wavy-convex (subtype I), shallow-basin (subtype II), and deep-hole (subtype III). Density of apical surfaces of mitochondrion-rich (MR) cell in gills of the BW-transfer tilapia decreased significantly within 3 h post-transfer due to disappearance of subtype I cells, but increased from 48 h post-transfer because of increasing density of subtype III cells. SW-transfer individuals, however, showed decreased density of MR cell openings after 1 h post-transfer because subtype I MR cell disappeared. On the other hand, relative branchial Na(+)/K(+)-ATPase (NKA) alpha 1-subunit mRNA levels, protein abundance, and NKA activity of the BW-transfer group increased significantly at 6,12, and 12 h post-transfer, respectively. In the SW-transfer group, relative mRNA and protein abundance of gill NKA alpha 1-subunit did not change while NKA activity declined before dying in 5 h. Upon SW transfer, dramatic increases (nearly 2-fold) of plasma osmolality, [Na(+)], and [Cl(-)] were found prior to death. For the BW-transfer group, plasma osmolality was eventually controlled by 96 h post-transfer by enhancement of NKA expression and subtype III MR cell. The success or failure of NKA activation from gene to functional protein as well as the development of specific SW subtype in gills were crucial for the survival of euryhaline tilapia to various hyperosmotic shocks. (C) 2008 Elsevier Inc. All rights reserved.
URI: http://hdl.handle.net/11455/37750
ISSN: 1095-6433
文章連結: http://dx.doi.org/10.1016/j.cbpa.2008.12.012
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