Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/37764
標題: The expression of gill Na, K-ATPase in milkfish, Chanos chanos, acclimated to seawater, brackish water and fresh water
作者: Lin, Y.M.
李宗翰
Chen, C.N.
Lee, T.H.
關鍵字: fresh water
brackish water
seawater
osmoregulation
gill
mitochondrion-tich cell
Na, K-ATPase
milkfish
mitochondria-rich cells
oreochromis-mossambicus
chloride cells
fundulus-heteroclitus
transport mechanisms
sea-water
adaptation
salinity
teleost
osmoregulation
期刊/報告no:: Comparative Biochemistry and Physiology a-Molecular & Integrative Physiology, Volume 135, Issue 3, Page(s) 489-497.
摘要: Juvenile milkfish Chanos chanos (Forssk (a) over circlel, 1775) were transferred from a local fish farm to fresh water (FW; 0parts per thousand), brackish water (BW, 10parts per thousand, 20parts per thousand) and seawater (SW; 35parts per thousand) conditions in the laboratory and reared for at least two weeks. The blood and gill of the fish adapted to various salinities were analyzed to determine the osmoregulatory ability of this euryhaline species. No significant difference was found in plasma osmolality, sodium or chloride concentrations of milkfish adapted to various salinities. In FW, the fish exhibited the highest specific activity of Na, K-ATPase (NKA) in gills, while the SW group was found to have the lowest. Relative abundance of branchial NKA alpha-subunit revealed similar profiles. However, in contrary to other euryhaline teleosts, i.e. tilapia, salmon and eel, the naturally SW-dwelling milkfish expresses higher activity of NKA in BW and FW. Immunocytochemical staining has shown that most Na, K-ATPase immunoreactive (NKIR) cells in fish adapted to BW and SW were localized to the filaments with very few on the lamellae. Moreover, in FW-adapted milkfish, the number of NKIR cells found on the lamellae increased significantly. Such responses as elevated NKIR cell number and NKA activity are thought to improve the osmoregulatory capacity of the milkfish in hyposaline environments. (C) 2003 Elsevier Science Inc. All rights reserved.
URI: http://hdl.handle.net/11455/37764
ISSN: 1095-6433
文章連結: http://dx.doi.org/10.1016/s1095-6433(03)00136-3
Appears in Collections:生命科學系所

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.