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|標題:||The expression of gill Na, K-ATPase in milkfish, Chanos chanos, acclimated to seawater, brackish water and fresh water|
|期刊/報告no：:||Comparative Biochemistry and Physiology a-Molecular & Integrative Physiology, Volume 135, Issue 3, Page(s) 489-497.|
|摘要:||Juvenile milkfish Chanos chanos (Forssk (a) over circlel, 1775) were transferred from a local fish farm to fresh water (FW; 0parts per thousand), brackish water (BW, 10parts per thousand, 20parts per thousand) and seawater (SW; 35parts per thousand) conditions in the laboratory and reared for at least two weeks. The blood and gill of the fish adapted to various salinities were analyzed to determine the osmoregulatory ability of this euryhaline species. No significant difference was found in plasma osmolality, sodium or chloride concentrations of milkfish adapted to various salinities. In FW, the fish exhibited the highest specific activity of Na, K-ATPase (NKA) in gills, while the SW group was found to have the lowest. Relative abundance of branchial NKA alpha-subunit revealed similar profiles. However, in contrary to other euryhaline teleosts, i.e. tilapia, salmon and eel, the naturally SW-dwelling milkfish expresses higher activity of NKA in BW and FW. Immunocytochemical staining has shown that most Na, K-ATPase immunoreactive (NKIR) cells in fish adapted to BW and SW were localized to the filaments with very few on the lamellae. Moreover, in FW-adapted milkfish, the number of NKIR cells found on the lamellae increased significantly. Such responses as elevated NKIR cell number and NKA activity are thought to improve the osmoregulatory capacity of the milkfish in hyposaline environments. (C) 2003 Elsevier Science Inc. All rights reserved.|
|Appears in Collections:||生命科學系所|
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