Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/37827
標題: Ambient salinity modulates the expression of sodium pumps in branchial mitochondria-rich cells of Mozambique tilapia, Oreochromis mossambicus
作者: Lee, T.H.
李宗翰
Feng, S.H.
Lin, C.H.
Hwang, Y.H.
Huang, C.L.
Hwang, P.P.
關鍵字: salinity
Na,K-ATPase
gill
mitochondria-rich cells
teleost
na-k-atpase
chloride cell
fresh-water
fundulus-heteroclitus
euryhaline teleost
gill arch
surface ultrastructure
opercular
epithelium
apical crypts
sea-water
期刊/報告no:: Zoological Science, Volume 20, Issue 1, Page(s) 29-36.
摘要: Na,K-ATPase (sodium pumps) provide the primitive driving force for ion transport in branchial epithelial cells. Immunoblots of epithelial homogenates of both seawater (SW)- and freshwater (FW)-adapted tilapia. gills as well as rat brain homogenate, a positive control, revealed one major band with a molecular weight of about 100 kDa. SW-adapted tilapia gills possessed larger (about 2-fold) amounts of sodium pumps compared with FW-adapted tilapia. gills. H-3-ouabain binding representing functional binding sites of Na,K-ATPase was also higher (about 3.5-fold) in gills of SW-adapted tilapia. compared to that of FW-adapted fish. Moreover, specific activities of SW fish were higher (about 2-fold) than those of FW fish. Double labeling of Na,K-ATPase and Con-A, a fluorescent marker of MR cells, in tilapia gills followed by analysis with confocal microscopy showed that sodium pumps were localized mainly in MR cells, including the SW type and different FW types. Although more-active expression of Na,K-ATPase was demonstrated in gills of SW-adapted tilapia, no significant differences in densities of apical openings of MR cells were found between SW- and FW-adapted fish. These results indicate that, during salinity challenge, tilapia develop more "functional" Na,K-ATPase in SW-type MR cells to meet physiological demands.
URI: http://hdl.handle.net/11455/37827
ISSN: 0289-0003
文章連結: http://dx.doi.org/10.2108/zsj.20.29
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