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|標題:||Na+,K+,2Cl(-)-cotransporter: A novel marker for identifying freshwater- and seawater-type mitochondria-rich cells in gills of the euryhaline tilapia, Oreochromis mossambicus|
|期刊/報告no：:||Zoological Studies, Volume 42, Issue 1, Page(s) 186-192.|
|摘要:||Yu-Ching Wu, Li-Yih Lin and Tsung-Han Lee (2003) Na+,K+,2Cl(-)-cotransporter: a novel marker for identifying freshwater- and seawater-type mitochondria-rich cells in gills of the euryhaline tilapia, Oreochromis mossambicus. Zoological Studies 42(1): 186-192. The abundance and location of the branchial Na+,K+,2Cl(-)-cotransporter (NKCC) were examined in freshwater- and seawater-adapted tilapia. Immunoblots revealed 4 bands with molecular masses centered at 282, 208, 122, and 105 kDa, respectively. Gill NKCC in tilapia is upregulated after seawater adaptation. Confocal laser scanning micrographs showed that in seawater-adapted tilapa, NKCC as well as Na,K-ATPase exhibited identical diffuse distribution confined to the basal portion of branchial mitochondria-rich cells. However, in freshwater-adapted tilapia, NKCC was displayed only in the apical region of Na,K-ATPase-immunoreactive cells. Polarized distribution of NKCC makes it a novel marker for recognizing freshwater- or seawater-type MR cells in euryhaline tilapia. http://www.sinica.edu.tw/zool/zoolstud/42.1/186.pdf.|
|Appears in Collections:||生命科學系所|
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