Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/37863
標題: Time-course changes in the expression of Na, K-ATPase and the morphometry of mitochondrion-rich cells in gills of euryhaline tilapia (Oreochromis mossambicus) during freshwater acclimation
作者: Lin, C.H.
李宗翰
Huang, C.L.
Yang, C.H.
Lee, T.H.
Hwang, P.P.
關鍵字: trout salmo-trutta
chloride cells
alpha-subunit
mozambique tilapia
fundulus-heteroclitus
atlantic salmon
na+/k+-atpase
salinity changes
gene-expression
messenger-rna
期刊/報告no:: Journal of Experimental Zoology, Volume 301A, Issue 1, Page(s) 85-96.
摘要: Changes in expression of Na, K-ATPase (NKA) and morphometry of mitochondrion-rich (MR) cells in gills of tilapia were investigated on a 96-hr time course following transfer from seawater (SW) to fresh water (FW). A transient decline in plasma osmolality and Na+, Cl- concentrations occurred from 3 hrs onward. Gills responded to FW transfer by decreasing NKA activity as early as 3 hrs from transfer. This response was followed by a significant decrease in the NKA isoform alpha1-mRNA abundance, which was detected by real-time PCR at 6 hrs post transfer. Next, a decrease of alpha1-protein amounts were observed from 6 hrs until 24 hrs post transfer. Additionally, during the time course of FW transfer, modifications in number and size of subtypes of gill MR cells were observed although no significant difference was found in densities of all subtypes of MR cells. These modifications were found as early as 3 hrs, evident at 6 hrs (exhibition of 3 subtypes of MR cells), and mostly completed by 24 hrs post transfer. Such rapid responses (in 3 hrs) as concurrent changes in branchial NKA expression and modifications of MR cell subtypes are thought to improve the osmoregulatory capacity of tilapia in acclimation from hypertonic SW to hypotonic FW. (C) 2004 Wiley-Liss, Inc.
URI: http://hdl.handle.net/11455/37863
ISSN: 0022-104X
文章連結: http://dx.doi.org/10.1002/jez.a.20007
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