請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/37881
標題: Cyclin-Dependent Kinase 5 Regulates Steroidogenic Acute Regulatory Protein and Androgen Production in Mouse Leydig Cells
作者: Lin, H.
赫, 林
Chen, M.C.
Ku, C.T.
關鍵字: expression
endocrine
cdk5
transcription
involvement
exocytosis
hormone
testis
star
p35
期刊/報告no:: Endocrinology, Volume 150, Issue 1, Page(s) 396-403.
摘要: The roles of cyclin-dependent kinase 5 (Cdk5) in central nervous system and neurodegenerative diseases have been intensely investigated in recent decades. Because protein expressions of Cdk5 and its regulator, p35, have been identified in Leydig cells, it is informative to further explore the novel function of Cdk5/p35 in male reproduction. Here we show that Cdk5/p35 protein expression and kinase activity in mouse Leydig cells are regulated by human chorionic gonadotrophin (hCG) in both dose-and time-dependent manners. Blocking of Cdk5 by molecular inhibitors or small interfering RNA resulted in reduction of testosterone production by Leydig cells. cAMP, a second messenger in LH signaling, was identified as a factor in hCG-dependent regulation of Cdk5/p35. Importantly, Cdk5 protein and kinase activity could support accumulation of steroidogenic acute regulatory (StAR) protein, a crucial component of steroidogenesis. We additionally addressed the protein interaction between Cdk5/p35 and StAR. The Cdk5-dependent serine phosphorylation of StAR indicated a possible mechanism by which Cdk5 induced accumulation of StAR protein. In conclusion, Cdk5 modulates hCG-induced androgen production in mouse Leydig cells, possibly through regulation of StAR protein levels. These results indicate that Cdk5 may play an important role in male reproductive endocrinology and is a potential therapeutic target in androgen-related diseases. (Endocrinology 150: 396-403, 2009)
URI: http://hdl.handle.net/11455/37881
ISSN: 0013-7227
文章連結: http://dx.doi.org/10.1210/en.2008-0496
顯示於類別:生命科學系所

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