Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3795
標題: 聚麩胺酸/脂質共組裝之酸鹼應答型奈米液胞性質探討
Characterization of pH-Responsive Nanovesicles from Assembly of Poly (γ-glutamic acid) with Cationic Lipids
作者: 莊宗憲
Chuang, Tsung-Hsien
關鍵字: vesicles
液胞
γ-glutamic acid
pH-Responsive
麩胺酸
酸鹼應答
出版社: 化學工程學系所
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摘要: 中文摘要 本研究利用生物可分解且無毒性的陰離子型高分子gamma-polyglutamic acid (γ-PGA)與一對疏水碳鏈之陽離子型界面活性劑1,2-Dioleoyl-3-Trimethylammonium-Propane (Chloride salt) (DOTAP)以靜電交互作用力相結合。實驗中利用動態和靜態光散射儀 (dynamic and static light scattering) 、螢光探針偵測 (fluorescence probe measurement) 、廣角X光繞射(Wide-Angle X-ray Diffraction, WAXD)以及穿透式電子顯微鏡(transmission electronic microscopy, TEM) 證實帶電高分子(γ-PGA)與帶相反電荷DOTAP形成結構穩定的複合液胞微粒。 調控高分子液胞之pH值,由SLS結果可知環動半徑(Rg)隨pH值變化而改變,說明了此液胞具有酸鹼應答特性。利用廣角X光繞射(wide-angle X-ray diffraction)結果可知液胞具有規則排列之脂雙層結構,另外,藉由螢光分光光譜儀測定疏水分子焦油腦(pyrene)之螢光強度比值,發現於低pH值下製備液胞,液胞表面有許多未解離且互相纏繞的γ-PGA,當調升pH值時,液胞表面γ-PGA逐漸解離而使脂雙層結構排列變的疏鬆,然而,於高pH值下製備液胞,液胞之脂雙層層間距並不隨pH值的變化而改變。 高分子液胞可穩定於水溶液中,因為液胞表面為解離帶電的γ-PGA,所以液胞之間有靜電排斥力(electrostatic repulsion),可穩定存在於水相中,不會有聚集和沉澱的現象發生。液胞具有親水區域的特性,可包覆親水性分子,以模擬液胞應用於藥物載體。
Abstract In this study, the complexes were prepared easily by the electrostatic interaction of the negatively charged biological gamma-Polyglumatic acid (γ-PGA) and double-tail cationic lipids 1,2-Dioleoyl-3-Trimethylammonium-Propane (Chloride salt) (DOTAP) in the aqueous solution. Combination of static light scattering (SLS)、wide-angle x-ray diffraction (WAXD) and transmission electronic microscopy (TEM) results, it is demonstrated that the complexes can assembly into the spherical vesicles structure.The difference of the structure of the vesicle assemblies is upon the starting solution pH. At low starting pH, the vesicle assemblies have the entanglement of the unionized γ-PGA chains on the surface of the vesicle assemblies. As the solution pH is increased, the bilayer structure becomes gradually looser due to the deprotonation of γ-PGA chains. Differently, the bilayer structure of the vesicle assemblies (at high starting pH) can not almost be affected while PGA chains are protonated. The vesicle assemblies can stable in the solution up to two weeks because the charged polymer chain layers are located in the outer reaches of vesicles to prevent the aggregation between the vesicle assemblies. The hydrophilic molecules can be encapsulated in the interior aqueous compartments to simulate the applications of drug delivery system.
URI: http://hdl.handle.net/11455/3795
其他識別: U0005-0508201017051000
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-0508201017051000
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