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|標題:||A water-based triphenyltetrazolium chloride method for the evaluation of green plant tissue viability|
|期刊/報告no：:||Phytochemical Analysis, Volume 12, Issue 3, Page(s) 211-213.|
|摘要:||A water-based 2,3,5-triphenyltetrazolium chloride (TTC) partitioning method using n-hexane for the evaluation of green plant tissue viability has been developed. Conventionally, the reduction of TTC to insoluble red-coloured triphenylformazan (TPF) has been used to detect seed, bud, leaf and cultured cell viability. However, the 95% ethanol used to extract TPF also extracts various pigments, such as chlorophyll, from plant tissues which interfere with the absorption of TPF at 485 nm. This new water-based method improves upon the current method by eliminating the interfering pigments in the hexane layer, and by minimising the non-enzymatic oxidation of the sample. When used to evaluate the tissue viability of chill-stressed waxapple (Syzygium samarangense) plants, the refined method had a greater sensitivity than the electrolyte leakage method and thus provided a more precise assessment for the physiological state of various plant tissues. Copyright (C) 2001 John Wiley & Sons, Ltd.|
|Appears in Collections:||生命科學系所|
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