Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/37989
標題: Constitutive expression of a fungal glucose oxidase gene in transgenic tobacco confers chilling tolerance through the activation of antioxidative defence system
作者: Maruthasalam, S.
林金和
Liu, Y.L.
Sun, C.M.
Chen, P.Y.
Yu, C.W.
Lee, P.F.
Lin, C.H.
關鍵字: Antioxidant
Chilling tolerance
Glucose oxidase
Hydrogen peroxide
Tobacco
oxidative stress tolerance
hydrogen-peroxide
disease resistance
cold-acclimation
maize seedlings
ascorbate peroxidase
superoxide-dismutase
signal-transduction
aspergillus-niger
abiotic
stress
期刊/報告no:: Plant Cell Reports, Volume 29, Issue 9, Page(s) 1035-1048.
摘要: Scientific evidences in the literature have shown that plants treated exogenously with micromole concentration of hydrogen peroxide (H(2)O(2)) acquire abiotic stress tolerance potential, without substantial disturbances in the endogenous H(2)O(2) pool. In this study, we enhanced the endogenous H(2)O(2) content of tobacco (Nicotiana tabaccum L. cv. SR1) plants by the constitutive expression of a glucose oxidase (GO; EC 1.1.3.4) gene of Aspergillus niger and studied their cold tolerance level. Stable integration and expression of GO gene in the transgenic (T(0)-T(2)) tobacco lines were ascertained by molecular and biochemical tests. Production of functionally competent GO in transgenic plants was confirmed by the elevated levels of H(2)O(2) in the transformed tissues. When three homozygous transgenic lines were exposed to different chilling temperatures for 12 h, the electrolyte conductivity was significantly lower in GO-expressing tobacco plants than the control plants; in particular, chilling protection was more prominent at -1A degrees C. In addition, most transgenic lines recovered within a week when returned to normal culture conditions after -1A degrees C-12 h cold stress. However, control plants displayed symptoms of chilling injuries such as necrosis of shoot tip, shoots and leaves, consequently plant death. The protective effect realized in the transgenic plants was comparable to cold-acclimatized wild tobacco. The chilling tolerance of transgenic lines was found associated, at least in part, with elevated levels of total antioxidant content, CAT and APX activities. Based on our findings, we predict that the transgenic expression of GO may be deployed to improve cold tolerance potential of higher plants.
URI: http://hdl.handle.net/11455/37989
ISSN: 0721-7714
文章連結: http://dx.doi.org/10.1007/s00299-010-0889-6
Appears in Collections:生命科學系所

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.