Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/38240
標題: Recombinant porcine lactoferrin expressed in the milk of transgenic mice enhances offspring growth performance
作者: 陳全木
Wu, S.C.
Chen, H.L.
Yen, C.C.
Kuo, M.F.
Yang, T.S.
Wang, S.R.
Weng, C.N.
Chen, C.M.
Cheng, W.T.K.
關鍵字: porcine lactoferrin
transgenic mice
mammary gland
growth rate
bovine lactoferrin
pichia-pastoris
antimicrobial activity
methylotrophic yeast
escherichia-coli
mammary-glands
gene
protein
cells
infection
期刊/報告no:: Journal of Agricultural and Food Chemistry, Volume 55, Issue 12, Page(s) 4670-4677.
摘要: The European Commission has proposed a permanent ban on the use of antibiotics as an ingredient in animal feed to promote growth. Lactoferrin is a globular multifunctional protein that has been shown to play a role in iron absorption and to have antimicrobial and anti-inflammatory activities. Therefore, lactoferrin may serve as a nontherapeutic alternative to antibiotics in livestock husbandry. As a pilot study toward this goal, transgenic mice have been generated harboring a porcine lactoferrin (pLF) gene driven by the mammary gland-specific promoter of the bovine alpha-lactalbumin (alpha LA) gene. The alpha LA-pLF hybrid gene was confirmed to have been successfully integrated and transmitted stably through the germ-line in 9 (5 females and 4 males) of 14 transgenic founders. In the female progenies of six lines analyzed, the transgene copy numbers ranged from 1 to 20 with 1-4 integration sites. Significant levels of pLF protein in milk ranging from 40 to 106 mu g/mL with physical characteristics similar to those of native pLF in sow's milk were achieved in three of the transgenic lines obtained. Tissue- and stage-specific pLF expressions were restricted to the mammary gland of the transgenic female mice during lactation. It was further demonstrated that the growth performance of animal pups is enhanced by directly feeding the genetically engineered milk containing enriched pLF protein in transgenic mice. Furthermore, this enhanced growth performance in suckling mice was proportional to the concentration of pLF present in milk.
URI: http://hdl.handle.net/11455/38240
ISSN: 0021-8561
文章連結: http://dx.doi.org/10.1021/jf063759o
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