Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/38247
標題: Association of creatin kinase B and peroxiredoxin 2 expression with age and embryo quality in cumulus cells
作者: 陳全木
Lee, M.S.
Liu, C.H.
Lee, T.H.
Wu, H.M.
Huang, C.C.
Huang, L.S.
Chen, C.M.
Cheng, E.H.
關鍵字: Aging
Creatin kinase B (CKB)
Cumulus cells
Embryo quality
Peroxiredoxin 2 (PRDX2)
companion somatic-cells
in-vitro fertilization
gene-expression
oocyte
quality
microarray analysis
predictive factors
hydrogen-peroxide
oxidative stress
tumor cytosols
isoenzyme
期刊/報告no:: Journal of Assisted Reproduction and Genetics, Volume 27, Issue 11, Page(s) 629-639.
摘要: The purpose of this study was to identify age-related oocyte or embryo markers suitable for non-invasive analysis, as women over 38 years of age experience diminished pregnancy and ovulation rates. We used real-time quantitative PCR to examine the gene expression profiles in cumulus cells acquired from older and younger age groups. We selected 11 genes involved in three functions that directly affect cellular aging: cell cycle control, apoptosis, and metabolism. CKB and PRDX2 were up-regulated in women older than 38 years, and the expression of these genes in cumulus cells was associated with embryo quality. In good-quality embryos, CKB expression was higher in the cumulus cells acquired from both older and younger age groups than in poor-quality embryos. These potential relationships among cumulus cell gene expression, oocyte quality, and age may expand our understanding of oogenesis and embryo development. CKB and PRDX2 may serve as biomarkers or therapeutic targets for the developmental potential of oocytes.
URI: http://hdl.handle.net/11455/38247
ISSN: 1058-0468
文章連結: http://dx.doi.org/10.1007/s10815-010-9459-7
Appears in Collections:生命科學系所

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