Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/38268
標題: Applications of CpG island microarrays for high-throughput analysis of DNA methylation
作者: 陳全木
Yan, P.S.
Chen, C.M.
Shi, H.D.
Rahmatpanah, F.
Wei, S.H.
Huang, T.M.H.
關鍵字: DNA methylation
CpG islands
microarray
methylenetetrahydrofolate reductase
breast-cancer
folic-acid
folate
risk
improvement
vitamin-b12
smokers
cells
gene
期刊/報告no:: Journal of Nutrition, Volume 132, Issue 8, Page(s) 2430S-2434S.
摘要: Differential methylation hybridization (DMH) is a high-throughput microarray technique designed to identify changes in DNA methylation patterns commonly observed in cancer and other disease states. The DMH methodology comprises three fundamental components: the arraying of CpG island clones on glass slides, the preparation of the sample amplicons under investigation, and the hybridization of amplicon targets onto the CpG island microarray. Herein, we outline the DMH protocol and illustrate its utility and the validation approaches used in analyzing the hypermethylation profile of breast tumor and normal samples.
URI: http://hdl.handle.net/11455/38268
ISSN: 0022-3166
Appears in Collections:生命科學系所

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.