Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/38312
標題: Splicing of a Bacterial Group II Intron from Bacillus megaterium Is Independent of Intron-Encoded Protein
作者: Chien, M.F.
黃介辰
Tosa, S.
Huang, C.C.
Endo, G.
關鍵字: group II intron
intron-encoded protein
splicing
maturase binding
rna
domain
identification
diversity
sediment
genes
ai2
dna
期刊/報告no:: Microbes and Environments, Volume 24, Issue 1, Page(s) 28-32.
摘要: The splicing of a bacterial group II subclass B intron B.me.I1 from Bacillus megaterium chromosomes was investigated. RT-PCR and nucleic acid hybridization methods were used to understand the role of the intron-encoded protein (IEP) in the splicing of B.me.I1. An in vivo assay showed that the splicing occurred in the absence of IEP. An in vitro assay showed that B.me.I1 was spliced under conditions similar to those of the intracellular environment with no help from other biological molecules. Because all group II introns previously reported needed IEPs for their splicing in vivo, our results suggest that B.me.I1 is an "actual" self-splicing group II intron. This is also the first report to recognize the existence of group II introns that independently splice mRNA in vivo. The self-splicing of a bacterial intron may support that eukaryotic spliceosomal introns originated in bacterial genomes.
URI: http://hdl.handle.net/11455/38312
ISSN: 1342-6311
文章連結: http://dx.doi.org/10.1264/jsme2.ME08540
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