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|標題:||Effects of sarcomere length and Ca2+ binding on SH reactivity of myofilament bound troponin C in porcine skinned cardiac muscle fibers|
|期刊/報告no：:||Japanese Journal of Physiology, Volume 51, Issue 3, Page(s) 385-388.|
|摘要:||Length dependence of cardiac Ca2+ activation is an essential component of the Frank-Starling relation. The aim of this study is to examine the length effects on the Ca2+-induced conformational changes of filament-bound cTnC in skinned cardiac muscle fibers. The two cysteine residues (Cys-35 and Cys-84) in the regulatory domain of cTnC allow for the attachment of conformational probes to this region. Their incorporation with the fluorescent probe, 7-diethylamino-3-[4 ' -maleimidylphenyl]-4-methylcoumarin (CPM), was used to determine the varying cTnC conformations in cardiac fibers. The data obtained show that the length-dependent Ca2+-mediated conformational changes require strong-binding cross-bridges for cardiac activation.|
|Appears in Collections:||生命科學系所|
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