Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/38391
標題: Binding potency of peptide fragments of type 1 collagen cross-linked N-telopeptide measured by an enzyme-linked immunosorbant assay
作者: Lung, F.D.T.
劉英明
Chen, C.H.
Chen, H.Y.
Liou, C.C.
Liou, Y.M.
關鍵字: osteoporosis
cross-linked N-telopeptide
type I collagen
synthetic
overlapping peptides
solid phase peptide synthesis
high performance
liquid chromatography
enzyme-linked immunosorbent assay
bone-resorption
i collagen
postmenopausal women
biochemical markers
alendronate
immunoassay
turnover
serum
urine
期刊/報告no:: Protein and Peptide Letters, Volume 9, Issue 5, Page(s) 451-457.
摘要: Osteoporosis represents a major healthcare problem affecting elderly person. Urinary level of the cross-linked N-telopeptide of type I collagen is a sensitive marker of bone resorption. Ten overlapping peptides covering the N-telopeptide of alpha-2 type I collagen were synthesized, purified, and assayed for their relative binding response to anti-type I collagen cross-linked N-telopeptide (NTX) antibody by using a competitive-inhibition enzyme-linked immunosorbent assay (ELISA). Peptides 1, 2, and 3, containing the N-terminal sequence of N-telopeptide, showed higher binding potency than peptides 4-10, suggesting that these peptides may contain binding sites for anti-NTX antibodies, and can serve as the lead for further preparation of their antibodies in order to develop novel bioassays for monitoring the bone loss in humans.
URI: http://hdl.handle.net/11455/38391
ISSN: 0929-8665
文章連結: http://dx.doi.org/10.2174/0929866023408544
Appears in Collections:生命科學系所

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