Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/38411
標題: Methanofollis formosanus sp nov., isolated from a fish pond
作者: Wu, S.Y.
賴美津
Chen, S.C.
Lai, M.C.
關鍵字: methanogenic bacteria
deoxyribonucleic-acid
estuarine environment
coccoid methanogen
gen. nov.
thermophilicum
marisnigri
emendation
olentangyi
liminatans
期刊/報告no:: International Journal of Systematic and Evolutionary Microbiology, Volume 55, Page(s) 837-842.
摘要: A mesophilic, hydrogenotrophic methanogen, strain ML15(T) was isolated from an aquaculture fish pond near Wang-gong, Taiwan. The cells were irregular cocci, non-motile, 1.5-2.0 mu m in diameter and Gram-negative. Cells of strain ML15(T) lysed easily in the presence of SDS (0.1 g l(-1)) and the S-layer protein had an M-r of 138800. The catabolic substrates utilized by this strain included formate and H-2/CO2, but not acetate, methanol, trimethylamine or secondary alcohols. Growth did not occur in minimal medium, but was observed when yeast extract and tryptone were added. Strain ML15(T) grew fastest at 37 degrees C, pH 6.6-7.0 and with 3% NaCl. Acetate was not required for cell growth. Trace amounts of tungstate promoted cell growth. The G+C contents of DNA of Methanofollis aquaemaris N2F9704(T) and strain ML15(T) were 59.1 and 58.4 mol%, respectively. Sequence analysis of the 16S rRNA genes of strain ML15(T) and selected Methanofollis species revealed similarities of 95-97%. Based on the data presented here, it is proposed that strain ML15(T) (= OCM 789(T) = DSM 15483(T)) represents a novel species, Methanofollis formosanus sp. nov.
URI: http://hdl.handle.net/11455/38411
ISSN: 1466-5026
文章連結: http://dx.doi.org/10.1099/ijs.0.63475-0
Appears in Collections:生命科學系所

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.