Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3842
標題: 聚乙烯醇固定化纖維素水解酵素的特性探討
Investigation on cellulase immobilization with Polyvinyl Alcohol
作者: 張蓉慈
Chang, Rong -Cih
關鍵字: cellullase
纖維素水解酵素
polyvinyl alcohol
glutaraldehyde
immobilization
crosslinking reaction
storage test
stability
聚乙烯醇
戊二醛
固定化
共價鍵結反應
儲存性
穩定
出版社: 化學工程學系所
引用: 江善宗、殷儷容,「纖維素分解酵素於綠藻工業之應用研究」,農業生技產業季刊,臺灣,第七期,2006。 田蔚城, 生物技術的發展與應用, 九州圖書文物有限公司,1998。 陳國誠,「生物固定化技術與產業應用」,茂昌圖書有限公司,臺灣,第48至57頁,1990。 井本三郎著、廖明隆譯 ,醋酸乙烯系塑膠,台灣文源書局有限公司,第77至126頁,1979。 薛敬和編 ,黏著劑全書- 材料與技術。台北高立圖書公司,第759至783頁,1985。 曾四恭,可同時硝化及脫硝之自營性薄膜生物反應槽之研發及其菌群結構分析,國立臺灣大學環境工程學研究所,2003。 曾政鴻,固定化乳酸菌香蕉發酵的研究,國立中興大學食品科學系博士論文,2003。 劉德亮,幾丁聚醣和聚乙烯醇摻合製備水膠膜之性質研究 國立台灣科技大學纖維及高分子工程系碩士論文,2000。 莊文源,聚乙烯醇薄膜結構形成機制的探討及其生醫材料應用的評估 國立台灣大學材料科學與工程學研究所碩士論文,1999。 方菘信 ,纖維水解酵素之固定化與其特性研究,大葉大學生物產業科技學系碩士論文,2007。 鍾志祥,探討固定纖維素水解酵素於幾丁聚醣擔體之研究,國立中央大學化學工程與材料工程研究所碩士論文,2007。 江衍徹,以雙重電性表面改質方式製作抗生物吸附之超過濾與奈米過濾膜,國立中央大學化學工程與材料工程研究所博士論文,2009。 宋憶青 ,溶膠-凝膠法應用於乙烯系樹脂/聚矽氧烷混成材料之基礎研究及其利用,國立中興大學森林學系博士論文,2009。 陳靜儀,固定化纖維素分解酵素於微藻細胞壁水解之研究,國立成功大學化學工程學系碩士論文,2008。 陳盛樂,真菌 Aspergillus terreus 固定化技術之探討---包埋法,朝陽科技大學應用化學系碩士論文,2004。 鐘國銘,利用反應曲面法探討幾丁聚醣-褐藻酸鈉複合膜之物化特性與其在中式香腸腸衣之應用,國立台灣海洋大學食品科學系碩士論文,2003。 Abdul, A.B. and Pethrick, R.A. (1982). "Ultrasonic studies of aqueous solutions of polyvinyl alcohol." Polymer 23: 1446-1450. Araujo, A.M., André, T.S., et al. (1996). "The Use of Polyvinyl Alcohol Glutaraldehyde as Solid-phase in ELISA for Plague.Mem Inst Oswaldo Cruz." Memórias do Instituto Oswaldo Cruz 91: 195-198. Araujo, A.M., Neves, M.T., et al. (1997). "Polyvinyl alcohol-glutaraldehyde network as a support for protein immobilisation." Biotechnology Techniques 11: 67-70 Belfort, G., Davis, R.H., et al. (1994). "The behavior of the suspensions and macromolecule solutions in crossflow microfiltration." Journal of Membrane Science 96: 1-58. Bhat, M.K. and Bhat, S. (1997). "Cellulose degrading enzymes and their potential industrial applications." Biotechnology advances 15: 583-620 Bickerstaff, G.F. (1997). "Immobilization of enzymes and cells: some practical considerations." Human Press: 1-11. Bottino, A., Capannelli, G., et al. (2005). "Novel porous poly (vinylidene fluoride)membranes for membrane distillation." Desalination 183: 375-382. Bradford, M.M. (1976). "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding." Analytical Biochemistry 72: 248-254. Bungay, H.R. (2004). "Confessions of a bioenergy advocate." Trends in Biotechnology 22: 67-71. Chang, C.L. and Chang, M.S. (2004). "Preparation of multi-layer silicone/PVDF composite membranes for pervaporation of ethanol aqueous solutions." Journal of Membrane Science 238: 117-122. Dincer, A. and Telefoncu, A. (2007). "Improving the stability of cellulase by immobilization on modified polyvinyl alcohol coated chitosan beads." Journal of Molecular Catalysis B: Enzymatic 45: 10–14. Diogo, M.M., Silva, S., et al. (1999). "Hydrophobic interaction chromatography of Chromobacterium viscosum lipase on polypropylene glycol immobilised on Sepharose." Journal of Chromatography A 849:413-419. Dohany, J.E. and Robb, L.E. (1980). "Polyvinylidene fluoride." Kirk-Othmer Encyclopedia of Chemical Technology 11: 64-74. Greg, T.H. (1996). "Bioconjugate techniques." Academic press. Gupta, S., Yogesh, et al. (2008). "Comparative study of performances of lipase immobilized asymmetric polysulfone and polyether sulfone membranes in olive oil hydrolysis." International Journal of Biological Macromolecules 42: 145-151. Gupta, Y., Hellgardt, K., et al. (2006). "Enhanced permeability of polyaniline based nano-membranes for gas separation." Journal of Membrane Science 282: 60-70. He, J., Li, X., et al. (2000). "A new support for the immobilization of penicillin acylase." Journal of Molecular Catalysis B: Enzymatic 11: 45-53. Hildén, L. and Johansson, G. (2004). "Recent developments on cellulases and carbohydrate-binding modules with cellulose affinity." Biotechnology Letters 26: 1683–1693. Jian, K. and Pintauro, P.N. (1997). "Asymmetric PVDF hollow-fiber membranes for organic/water pervaporation separations." Journal of Membrane Science 135: 41-53. Jim, K.J. (1992). "Fouling mechanisms of membranes during protein ultrafiltration." Journal of Membrane Science 68: 79-91. King, K. (1994). "Changes in the functional properties and molecular weight of sodium alginate following irradiation." Food Hydrocolloid 8: 83-96. Kotha, A., Selvaraj, L., et al. (1991). "Adsorption and expression of penicillin G acylase immobilized onto methacrylate polymers generated with varying pore generating solvent volume." Applied Biochemistry and Biotechnology 30: 297-302. Martinek, K., Klibanov, A.M., et al. (1997). "The principles of enzyme stabilization 1.Increase in thermostability of enzymes covalently bound to a complementary surface of a polymer support in a multipoint fashio." Biochimica Et Biophysica Acta Enzymology 485: 1-12. Miller, G.L. (1959). "Use of dinitrosalicylic acid reagent for determination of reducing sugar." Analytical Chemistry 31: 426-428. Moe, S.T., Draget, K.I., et al. (1995). "Alginates. Food Polysaccharides and Their Application." 245-286. Naidja, A., Huang, P.M., et al. (1997). "Activity of tyrosinase immobilized on hydroxyaluminum-montmorillonite complexes " Journal of Molecular Catalysis A: Chemical 115: 305-316. Norde, W. and Haynes, C.A. (1995). "Reversibility and the mechanism of protein adsorption." American Chemical Society 602: 27-40. Rhiam, J.W., Yeom, C.K., et al. (1998). "Modification of poly (vinyl alcohol) membranes using sulfur-succinic acid and its application to pervaporation separation of water–alcohol mixtures." Journal of Applied Polymer Science 68: 1717-1723. Soresi, B. (2004). "PVDF and P(VDF-HFP)-based proton exchange membranes." Solid State Ionics 166: 383-392. Souzy, R. and Ameduri, B. (2005). "Functional fluoropolymers for fuel cell membranes." Progress in Polymer Science 30: 644-652. Srisurichan, S., Jiraratananon, R., et al. (2006). "Mass transfer mechanisms and transport resistances in direct contact membrane distillation process." Journal of Membrane Science 277: 186-194. Sun, H.X. (2006). " A study of human γ-globulin adsorption capacity of PVDF hollow fiber affinity membranes containing different amino acid ligands." Separation Purification Technology 48: 215-222. Tomaz, C.T., Duarte, D., et al. (2002). "Comparative study on the fractionation of cellulases on some hydrophobic interaction chromatography adsorbents." Journal of Chromatography A 944: 211-216. Tsai, Y.H., Wang, M.Y., et al. (2002). "Purification of hepatocyte growth factor using polyvinyldiene fluoride-based immobilized metal affinity membranes: equilibrium adsorption study." Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 766: 133-143. Wu, L., Yuan, X., et al. (2005). "Immobilization of cellulase in nanofibrous PVA membranes by electrospinning ."Journal of Membrane Science 250: 167-173. Zou, H., Luo, Q., et al. (2001). "Affinity membrane chromatography for the analysis and purification of proteons." Journal of Biochemical and Biophysical Methods 49: 199-240.
摘要: 本研究測試不同固定化方法來固定化纖維素水解酵素,以(regenerated cellulose membrane,RC膜)、玻璃纖維膜(glass fiber membrane,GFM膜)、聚乙烯醇膜(polyvinyl alcohol membrane,PVA膜 )、聚偏二氟乙烯膜 (poly vinylidene fluoride,PVDF膜)、聚醚碸膜(polyether sulfone,PES膜)鍵結戊二醛,再以戊二醛來和酵素上的NH2形成共價鍵結。其中再生纖維膜會被纖維素水解酵素分解;而疏水性聚偏二氟乙烯膜、聚醚碸膜,使基質無法靠近酵素活性位置,因此均不適合固定化纖維素水解酵素;玻璃纖維膜及聚乙烯醇膜只能循環使用三次;聚乙烯醇和褐藻膠以玻璃纖維膜包埋法,所形成的膜結構不穩定,也只能循環使用三次。 使用PVA共價鍵結包埋纖維素水解酵素,其固定化效果最好,以戊二醛當PVA交聯劑,包埋酵素並鍵結酵素上的NH2官能基,6%戊二醛所形成之固定化膜循環使用可達10次,其相對活性仍可維持在18.45%,在pH 7磷酸緩衝溶液儲存四天其相對活性可維持在52.95%。
In this study,various matrix inclubing regenerated cellulose membrane(RCM),glass fiber membrane(GFM), polyvinyl alcohol membrane(PVAM), poly vinylidene fluoride membrane (PVDFM)、polyether sulfone membrane (PESM) were used for cellulase immobilization.Glutaraldehyde was used as the crosslinking reagent coupling with the amine of cellulase.Among them,RCM gave the highest cellulase activity,whereas,it will lose its activity rapidly due to the digestion of cellulase on the RCM.Both PVDFM and PESM are improper matrix for cellulase immobilization due to the hydrophobic characteristics.Though GFM is capable of coupling cellulase,its structure is rather agile in aqueous solution. Polyvinyl alcohol as the matrix for cellulase immobilization can endure more than 3 times repeated use was chosen as the matrix for further study. In this study, it is found that polyvinyl alcohol along with glutaraldehyde as the covalent reagent to simultaneously entrap and bind cellulase gave the best result for cellulase immobilization.When using 6% glutaraldehyde as the reagent,the PVA membrane was structurally stable in aqueous solution.In the stability test, the PVA-immobilized cellulase can undergo more than 10 times repeated use with 18.45% residual activity retained.In the storage test,the polyvinyl alcohol membrane immersed in pH 7 phosphate buffer can maintained 52.95% of its activity after 4 –day storage.
URI: http://hdl.handle.net/11455/3842
其他識別: U0005-2408201016464900
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2408201016464900
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