請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/38508
標題: Structure of Stenotrophomonas maltophilia FeoA complexed with zinc: a unique prokaryotic SH3-domain protein that possibly acts as a bacterial ferrous iron-transport activating factor
作者: Su, Y.C.
周三和
Chin, K.H.
Hung, H.C.
Shen, G.H.
Wang, A.H.J.
Chou, S.H.
洪慧芝
關鍵字: diphtheria-toxin repressor
sh3 domains
crystal-structure
recognition
reveals
binding
determinants
resistance
pathogen
system
期刊/報告no:: Acta Crystallographica Section F-Structural Biology and Crystallization Communications, Volume 66, Page(s) 636-642.
摘要: Iron is vital to the majority of prokaryotes, with ferrous iron believed to be the preferred form for iron uptake owing to its much better solubility. The major route for bacterial ferrous iron uptake is found to be via an Feo (ferrous iron-transport) system comprising the three proteins FeoA, FeoB and FeoC. Although the structure and function of FeoB have received much attention recently, the roles played by FeoA and FeoC have been little investigated to date. Here, the tertiary structure of FeoA from Stenotrophomonas maltophilia (Sm), a vital opportunistic pathogen in immunodepressed hosts, is reported. The crystal structure of SmFeoA has been determined to a resolution of 1.7 angstrom using an Se single-wavelength anomalous dispersion (Se-SAD) approach. Although SmFeoA bears low sequence identity to eukaryotic proteins, its structure is found to adopt a eukaryotic SH3-domain-like fold. It also bears weak similarity to the C-terminal SH3 domain of bacterial DtxR (diphtheria toxin regulator), with some unique characteristics. Intriguingly, SmFeoA is found to adopt a unique dimer cross-linked by two zinc ions and six anions (chloride ions). Since FeoB has been found to contain a G-protein-like domain with low GTPase activity, FeoA may interact with FeoB through the SH3-G-protein domain interaction to act as a ferrous iron-transport activating factor.
URI: http://hdl.handle.net/11455/38508
ISSN: 1744-3091
文章連結: http://dx.doi.org/10.1107/s1744309110013941
顯示於類別:生命科學系所

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