Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/38538
DC FieldValueLanguage
dc.contributor.authorShen, C.I.en_US
dc.contributor.author蘇鴻麟zh_TW
dc.contributor.authorWang, C.H.en_US
dc.contributor.authorLiao, J.W.en_US
dc.contributor.authorHsu, T.W.en_US
dc.contributor.authorKuo, S.M.en_US
dc.contributor.authorSu, H.L.en_US
dc.contributor.author廖俊旺zh_TW
dc.date2010zh_TW
dc.date.accessioned2014-06-06T08:00:54Z-
dc.date.available2014-06-06T08:00:54Z-
dc.identifier.issn0928-4249zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/38538-
dc.description.abstractHere we introduce a culture system for the isolation, passaging and amplification of avian tracheal epithelial (ATE) cells. The ATE medium, which contains chicken embryo extract and fetal bovine serum, supports the growth of ciliated cells, goblet cells and basal cells from chicken tracheas on fibronectin- or matrigel-coated dishes. Non-epithelial cells make up less than 10% of the total population. We further show that ATE cells support the replication and spread of infectious bronchitis virus (IBV). Interestingly, immunocytostaining revealed that basal cells are resistant to IBV infection. We also demonstrate that glycosaminoglycan had no effect on infection of the cells by IBV. Taken together, these findings suggest that primary ATE cells provide a novel cell culture system for the amplification of IBV and the in vitro characterization of viral cytopathogenesis.en_US
dc.language.isoen_USzh_TW
dc.relationVeterinary Researchen_US
dc.relation.ispartofseriesVeterinary Research, Volume 41, Issue 1.en_US
dc.relation.urihttp://dx.doi.org/10.1051/vetres/2009054en_US
dc.subjecttracheal epithelial cellen_US
dc.subjectinfectious bronchitis virusen_US
dc.subjectbasal cellen_US
dc.subjectprimary cultureen_US
dc.subjectglycosaminoglycanen_US
dc.subjectinfluenza-a virusesen_US
dc.subjectbasal-cellsen_US
dc.subjectsialic-aciden_US
dc.subjectwaterfowlen_US
dc.subjectchickensen_US
dc.subjectdifferentiationen_US
dc.subjectpathogenicityen_US
dc.subjectstrainsen_US
dc.subjectmultipotenten_US
dc.subjectadaptationen_US
dc.titleThe infection of primary avian tracheal epithelial cells with infectious bronchitis virusen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1051/vetres/2009054zh_TW
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