Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/38548
標題: Spinal glutamatergic NMDA-dependent pelvic nerve-to-external urethra sphincter reflex potentiation caused by a mechanical stimulation in anesthetized rats
作者: Liao, J.M.
董光中
Huang, P.C.
Pan, S.F.
Chen, M.J.
Tung, K.C.
Peng, H.Y.
Shyu, J.C.
Liou, Y.M.
Chen, G.D.
Lin, T.B.
劉英明
關鍵字: long-term potentiation
lower urinary-tract
micturition reflex
synaptic-transmission
receptor antagonists
alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid
lasting
potentiation
bladder contraction
dorsal horn
cord-injury
期刊/報告no:: American Journal of Physiology-Renal Physiology, Volume 292, Issue 6, Page(s) F1791-F1801.
摘要: The current study investigates whether the spinal pelvic nerve-to-external urethra sphincter (EUS) reflex potentiation can be induced by a mechanical stimulation and whether the glutamatergic mechanism is involved in yielding such a reflex potentiation. The external urethra sphincter electromyogram (EUSE) activity, evoked by a single or by repetitive pelvic nerve stimulation, in 30 anesthetized rats was recorded with/without bladder saline distension. Without saline distension (0 cmH(2)O), a single pulse nerve stimulation evoked a single action potential in the reflex activity, whereas repetitive pelvic stimulation and saline distension (6 similar to 20 cmH(2)O) both elicited a long-lasting reflex potentiation (20.05 +/- 3.21 and 75.01 +/- 9.87 spikes/stimulation, respectively). The saline distension-induced pelvic nerve-to-EUS reflex potentiation was abolished by D-2-amino-5-phosphonovalerate [APV; a glutamatergic N-methyl-D-aspartic acid ( NMDA) receptor antagonist; 100 mu M, 10 mu l, 1.72 +/- 0.31 spikes/stimulation] and attenuated by 2,3-dihydroxy-6-nitro-7-sulfamoylbenzo ( F) quinoxaline [ NBQX; a glutamatergic alpha-amino-3-hydroxy-5-methyl-4- isoxazoleproprionate ( AMPA) receptor antagonist; 100 mu M, 10 mu l, 26.16 +/- 7.27 spikes/stimulation], but was not affected by bicuculline (a GABAergic antagonist; 100 mu M, 10 mu l, 53.62 +/- 15.54 spikes/stimulation). Intrathecal administration of glutamate (31.12 +/- 8.25 spikes/stimulation, 100 mu M, 10 mu l) and NMDA (26.25 +/- 4.12 spikes/stimulation, 100 mu M, 10 mu l) both induced a long-lasting pelvic nerve-to-EUS reflex potentiation without saline distension, which was similar to the findings observed from saline distension only. The duration of the contraction wave of the urethra was elongated by the saline distension-induced pelvic nerve-to-EUS reflex potentiation, whereas the peak pressure of the contraction wave was not affected. Our findings suggest that saline distension in the bladder elicits a pelvic nerve-to-EUS reflex potentiation and the glutamatergic mechanism contributes to the presence of such a reflex potentiation.
URI: http://hdl.handle.net/11455/38548
ISSN: 1931-857X
文章連結: http://dx.doi.org/10.1152/ajprenal.00443.2006
Appears in Collections:生命科學系所

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