Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3884
標題: 多層壁奈米碳管/全氟磺酸聚合物複合薄膜作為電化學核酸適合體感測器之探討
Electrochemical aptamer sensors based on multiwalled carbon nanotube/Nafion composite film
作者: 陳惠鈞
Chen, Hui-Chun
關鍵字: carbon nanotube
核酸適合體
aptamer
adenosine
腺苷
出版社: 化學工程學系所
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摘要: 本研究成功使用單股DNA(single-stranded DNA, capture probe),核酸適合體(aptamer),多層壁奈米碳管(multi-walled carbon nanotube, MWCNT),全氟黃酸聚合物(Nafion),製備出aptamer/capture probe/MWCNT/Nafion薄膜之電化學核酸適合體感測器,並證明具有偵測腺苷(adenosine)的能力。以循環伏安法(CV)觀察電極電化學特徵變化,並使用原子力顯微鏡(atomic force microscope, AFM)觀察所製備薄膜之表面形態與方均根粗糙度(root-mean-square roughness, RMS)改變,證明核酸適合體成功固定在電極上。當aptamer/capture/MWCNT/Nafion電極與腺苷反應後,透過微分脈衝伏安法(DPV)之波峰電流觀察[Fe(CN)6]3-在電極表面的質傳或電子傳遞的變化,進而得到不同濃度腺苷的訊號改變。為了使核酸適合體感測器之表現最佳化,我們探討幾個實驗條件的最適值如雜交時間、腺苷與其適合體反應時間等。本研究在最佳化後之腺苷適合體感器的線性範圍為10-5到 10-9M,並得靈敏度為21.93 (µA/cm2*log mol/L),偵測極限為8×10-10M。
A label-free electrochemical aptasensor for the detection of adenosine based on multi-walled carbon nanotube (MWCNT)/Nafion was presented. The capture probe which amine modified was covalently conjugated to modified electrode. The aptamer was immobilized on the electrode by hybridization reaction with capture probe. In the present of adenosine, the aptamer folded to the complex structure with adenosine and subsequent dissociation of the adenosine-aptamer complex from the electrode surface. To further confirm immobilization of capture probe and aptmaer, the morphology of the resulting MWCNT/Nafion/GCE, capture probe/ MWCNT/Nafion/GCE, and aptamer/capture probe/MWCNT/Nafion/GCE were investigated by atomic force microscopy (AFM). The increased RMS value indicated that the capture probe and aptmer were immobilized on the electrode. The variation of peak current of the adenosine with different concentrations was detected by differential pulse voltammetry (DPV) using [Fe(CN)6]3- as mediator. After the optimized experimental conditions, the increasing peak current of [Fe(CN)6]3- was in proportion the concentration of adenosine in the range from 10-5 to 10-9 M with a detection limit of 8×10-10M.
URI: http://hdl.handle.net/11455/3884
其他識別: U0005-1107201113380600
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-1107201113380600
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