Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3958
標題: 利用飛秒雷射製作2D柱狀PLGA微血管支架與中空3D PLGA支架
Fabrication of pillared 2D microvessel scaffold and hollow 3D scaffold on PLGA using femtosecond laser ablation
作者: 王曉威
Wang, Hsiao-Wei
關鍵字: 柱狀結構之PLGA人工微血管支架
Pillared PLGA microvessel scaffold
中空3D PLGA支架
飛秒雷射剝除系統
內皮細胞培養
hollow 3D PLGA scaffold
femtosecond laser ablation
Cell culture of endothelial cells
出版社: 生醫工程研究所
引用: [1]宋信文、梁晃千,“建立人類的人體工房-組織工程”,科學發展 Vol. 362, 6-11, 2003。 [2]Madou MJ, Lee LJ, Daunert S, Lai Sa and Shih CH, “Design and Fabrication of CD-like Microfluidic Platforms for Diagnostics: Microfluidic Functions.” Biomedical Microdevices, 3(3):245-254, 2003. [3]Martin RS, Gawron AJ, Lunte SM and Henry CS, “Dual-Electrode Electrochemical Detection for Poly(dimethylsiloxane)-Fabricated Capillary Electrophoresis Microchips.” Analytical Chemistry, 72(14):3196-3202, 2000. [4]Jiang G, Attiya S, Ocvirk G, Lee WE and Harrison DJ, “Red diode laser induced fluorescence detection with a confocal microscope on a microchip for capillary electrophoresis.” Biosensors and Bioelectronics, 14(10-11):861-869, 2000. [5]Polla DL, Krulevitch P, Wang A, Smith G, Diaz J, Mantell S, Zhou J, Zurn S, Nam Y, Cao L, Hamilton J and Fuller C, “Gascoyne P. MEMS–based diagnostic Microsystems. Proceeding of the 1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies.” Medicine & Biology, 41-44,2000. [6]Alvarez M, Calle A, Tamayo J, Lechuga LM and Abad A, “Montoya A. Development of nanomechanical biosensors for detection of the pesticide DDT.” Biosensors and Bioelectronics, 18(5-6):649-653, 2003. [7]Fidkowski C, Kaazempur-Mofrad M, Borenstein J, Vacanti JP, Langer R and Wang Y, “Endothelialized Microvasculature Based on a Biodegradable Elastomer.” Tissue Engineering, 11(1-2):302-309, 2005. [8]Wang GJ, Hsu YF, Hsu SH and Horng RH, “JSR Photolithography Based Microvascular Fabrication and Cell Seeding.” Biomedical Microdevices, 8(1):17-28, 2006. [9]Kovacs GTA, Maluf NI and Petersen KE, “Bulk micromachining of silicon.” Proceedings of the IEEE, 86(8):1536-1551, 1998. [10]Pan LC, Liang YC, Tseng FG, Leou KC, Chen LD and Lai YY, “A novel application of acoustic plate mode sensor in tissue regeneration, ”Proceedings of the IEEE-EMBS Special Topic Conference on Microtechnologies.” Medicine & Biology, 143-144, 2002. [11]Chang HK and Kim YK, “UV-LIGA process for high aspect ratio structure using stress barrier and C-shaped etch hole.” Sensors and Actuators, 84(3):342-350, 2000. [12]Jeon NL and Chiu DT, “Microfluidics Section: Design and Fabrication of Integrated Passive Valves and Pumps for Flexible Polymer 3-Dimensional Microfluidic Systems.” Biomedical Microdevices, 4(2):117-121, 2002. [13]Kancharla VV and Chen S, “Fabrication of Biodegradable Polymeric Micro-Devices Using Laser Micromachining.” Biomedical Microdevices, 4(2):105-109, 2002. [14]Ahn CH, Chol JW, Beaucage G, Nevin JH and Lee JB, “Puntambekar A and Lee JY. Disposable smart lab on a chip for point-of-care clinical diagnostics.” Proceedings of the IEEE, 92(1):154-173, 2004. [15]McCormick RM, Nelson RJ, Alonso-Amigo MG, Benvegnu DJ and Hooper HH, “Microchannel electrophoretic separations of DNA in injection-molded plastic substrates.” Analytical Chemistry, 69:2626-2630, 1997. [16]Xia Y and Whitesides GM, “Soft Lithography.” Annual Review of Materials Science, 28: 153-184, 1998. [17]Rogers JA and Nuzzo RG, “Recent progress in soft lithography.” Materials today, 8:50-56, 2005. [18]Borenstein J, Terai H, King KR, Weinberg EJ and Vacanti JP, “Microfabrication technology for vascularized tissue engineering.” Biomedical Microdevices, 4(3): 167-175, 2002. [19]Shin M, Matsuda K, Ishii O, Terai H, Kaazempur-Mofrad M, Borenstein J, Detmar M and Vacanti JP, “Endothelialized Networks with a Vascular Geometry in Microfabricated Poly (dimethyl siloxane).” Biomedical Microdevices, 6(4): 269-278, 2004. [20]Wang GJ, Chen CL, Hsu SH and Chiang YL, “Bio-MEMS Fabricated Artificial Vascular Network for Tissue Engineering.” Microsystem Technologies, 12(1-2):120-127, 2005. [21]Wang GJ and Hsu YF, “Structure Optimization of Microvascular Scaffolds.” Biomedical Microdevices, 8(1):51-58, 2006. [22]Wang GJ, Hsu YF, Hsu SH and Horng RH, “JSR Photolithography Based Microvascular Fabrication and Cell Seeding.” Biomedical Microdevices, 8(1):17-28, 2006. [23]Yang LJ, Chen YT, Kang SW and Wang YC, “Fabrication of SU-8 embedded microchannels with circular cross-section.” International Journal of Machine Tools & Manufacture, 44:1109-1114, 2004. [24]Yi Y, Kang JH and Park JK, “Moldless electroplating for cylindrical microchannel fabrication.” Electrochemistry Communications, 7:913-917, 2005. [25]King KR, Wang CCJ, Kaazempur-Mofrad MR, Vacanti JP and Borenstein JT, “Biodegradable microfludics.” Advenced Materials, 16(22):2007-2012, 2004. [26]Vozzi G, Flaim C, Ahluwalia A and Bhatia S, “Fabrication of PLGA scaffolds using soft lithography and microsyringe deposition.” Biomaterials, 24(14):2355-2540, 2003. [27]Lu Y and Chen SC, “Micro and nano-fabrication of biodegradable polymers for drug delivery.” Advanced Drug Delivery Reviews, 1621-1633, 2004. [28]Wang GJ, Hsueh CC, Hsu SH and Hung HS, “Fabrication of PLGA Microvessel Scaffolds with Circular Microchannels Using Soft Lithography.” Journal of Micromechanics and Microengineering, 17:2000-2005, 2007. [29]Park GE, Park K and Webster TJ, “NaOH-Treated PLGA Scaffolds Allow for GreaterArticular Chondrocyte Functions.” Biomaterials, 26(16):3075-3082, 2005. [30]Savaiano JK and Webster TJ, “Altered responses of chondrocytes to nanophase PLGA/nanophase titania composites.” Biomaterials, 25:1205-1213, 2004. [31]Webster TJ, Tong Z, Liu J and Banks MK, “Adhesion of Pseudomonas fluorescens onto nanophase materials.” Nanotechnology, 16:S449-S457, 2005. [32]Miller DC, Haberstroh KM and Webster TJ, “PLGA nanometer surface features manipulate fibronectin interactions for improved vascular cell adhesion.” Journal of Biomedical Materials Research Part A, 81A(3):678-684, 2007. [33]Min BM, You Y, Kim JM, Lee SJ and Park WH, “Formation of nanostructured poly(lactic-co-glycolic acid)/chitin matrix and its cellular response to normal human keratinocytes and fibroblasts.” Carbohydrate Polymers, 57:285-292, 2004. [34]Wang GJ, Lin YC and Hsu Sh, “The fabrication of PLGA microvessel scaffolds with nano-patterned inner walls.” Biomedical Microdevices, 12(5):841-848, 2010. [35]Malek CK, Robert L and Salut R, “Femtosecond laser machining and lamination for large-area flexible organic microfluidic chips.” The European Physical Journal Applied Physics, 46:12503-12507, 2011. [36]Kruger J and Kautek W, “The Femtosecond Pulse Laser: a New Tool for Micromachining.” Laser Physics, 9(1):30-40, 1999. [37]Chimmalgi A, Choi TY, Grigoropoulos CP and Komvopoulos K, “Femtosecond laser aperturless near-field nanomachining of metals assisted by scanning probe microscopy.” Applied Physics Letters, 82(8):1146-1148, 2003. [38]Cheng CW, Shen WC, Lin CY, Lee YJ and Chen JS, “Fabrication of micro/nano crystalline ITO structures by femtosecond laser pulses.” Applied Physics A, 101:243-248, 2010 [39]Kamata M, Obara, M Gattass, RR, Cerami LR and Mazur E, “Optical vibration sensor fabricated by femtosecond laser micromachining.” Applied Physics Letters, 87(5):051106-051108, 2005. [40]Maselli V, Osellame R, Cerullo G, Ramponi R, Laporta P, and Cavallotti PL, “Fabrication of long microchannels with circular cross section using astigmatically shaped femtosecond laser pulses and chemical etching.” Applied Physics Letters, 88(19):191107-191109, 2006. [41]Chung SH, Clark DA, Gabel CV, Mazur E and Samuel ADT, “The role of the AFD neuron in C-elegans thermotaxis analyzed using femtosecond laser ablation.” BMC Neuroscience, 7:30, 2006. [42]Gaspard S, Forster M and Huber C, “Femtosecond laser processing of biopolymers at high repetition rate.” Physical Chemistry Chemical Physics, 10:6174-6181, 2008. [43]An R, Uram JD, Yusko EC, Ke K, Mayer M and Hunt AJ, “Ultrafast laser fabrication of submicrometer pores in borosilicate glass.” Optics Letters, 33(10):1153-1155, 2008. [44]Vorobyev AY, Makin VS and Guo C, “Brighter Light Sources from Black Metal: Significant Increase in Emission Efficiency of Incandescent Light Sources.” Physical Review Letters, 102(23):234301-234303, 2009. [45]Lee CY, Chang TC, Wang SC, Chien CW and Cheng CW, “Using femtosecond laser to fabricate highly precise interior three dimensional micro-structures in polymeric flow chip.” Biomicrofluidics, 4:046502, 2010. [46]Chen CH, Chao TC, Li WY, Shen WC, Tang JL, Jen CP, Cheng CW, Chau LK and Wu WT, “Novel U-shape gold nanoparticles-modified optical fiber for localized plasmon resonance sensing.” Microsystem Technologies, 16(7):1207-1214, 2010. [47]Cheng CW, Lee YJ, Shen WC, Chen JS and Chien CW, “Femtosecond laser-induced crystallization of amorphous indium tin oxide film on glass substrate for patterning applications.” Journal of Laser Micro Nanoengineering, 4:165-169, 2009. [48]Yong CL, Jed J, Zhengzheng F, Yun W, Dave F, John L, Hae WC and James L, “Micropatterning and Characterization of Electrospun Poly(ε-Caprolactone)/Gelatin Nanofiber Tissue Scaffolds by Femtosecond Laser Ablation for Tissue Engineering Applications.” Biotechnology and Bioengineering, 108(1):116-126, 2011. [49]李怡閔,“不同飛秒雷射波長對氧化銦錫加工結果探討”,國立中正大學工學院機械工程學系碩士論文,2010。 [50]丁勝懋,“雷射工程導論”,中央圖書出版社出版,2006。
摘要: The key issues involved in tissue engineering are how to culture specific cells on a suitable scaffold and to provide a satisfactory growth factor to regulate the differentiation and proliferation of the cells. Scaffolds function as the base for cell adhesion and migration, the place for the exchange of nutrients, and to deliver and retain cells and biochemical factors. In this study, the femtosecond laser ablation technique was implemented for the fabrication of 2D pillared microvessel scaffolds of polylactic-co-glycolic acid (PLGA) and hollow 3D PLGA scaffolds. For the 2D pillared microvessel scaffolds, PLGA scaffolds consisting of 47 μm × 80 μm pillared branches were produced. Results of cell culturing of bovine endothelial cells (BECs) demonstrate that the cells adhere well and grow to surround each branch of the proposed pillared microvessel networks. This novel scaffold facilitates implementation of the conventional cell seeding process. The progress of cell growth can be observed in vitro by optical microscopy. The problems of becoming milky or completely opaque with the conventional PLGA scaffold after cell seeding can be resolved. In this study, For the hollow 3D PLGA scaffolds, a salt ingot which was used as a temporary frame to define the shape of the desired scaffold was fabricated by extrusion molding. The salt ingot was then immersed in a PLGA solution and allowed to be entirely enveloped by the PLGA. The femtosecond laser ablation technique was used for direct writing of the desired pattern on the PLGA layer and finally the salt ingot inside was completely dissolved in distilled deionized water to obtain a hollow 3D PLGA scaffold on which BECs were then cultured. The cell culturing results are illustrated by SEM and fluorescent images and demonstrate that the BECs could adhere well and proliferate on the branches of the hollow 3D PLGA scaffold.
URI: http://hdl.handle.net/11455/3958
其他識別: U0005-1807201212372200
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-1807201212372200
Appears in Collections:生醫工程研究所

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.