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標題: 葡萄糖氧化酵素型生物燃料電池之最佳化特性研究
Optimization of Enzymatic Bio-Fuel Cell for Immobilization of Glucose Oxidase on Chitosan Coated Carbon Cloth
作者: 黃暐翔
Huang, Wei-Hsiang
關鍵字: Enzymatic Bio-Fuel Cell
Covalent ImmobilizingResponse Surface Methodology
出版社: 精密工程學系所
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摘要: 本研究的目標為開發酵素型生物燃料電池系統並進行電池特性之探討,達到電池效能提升之目的。其酵素電池的陽極以葡萄糖氧化酵素作為觸媒,使用共價鍵結的方式將酵素固定在具有幾丁聚醣擔體之碳布上,以反應曲面法探討其固定化時間、固定化溶液之pH值、酵素/擔體比值對活性之影響,並以統計方法找出預測其活性之反應曲面迴歸方程式。然後利用陽極葡萄糖量的改變與酵素活性的不同進行發電量的探討。其結果發現,酵素活性與葡萄糖量皆為造成發電量的重要因素。當陽極溶液之葡萄糖量為0.1M、活性34.4U/g時有較佳的發電特性,其最大發電量為1.672mW/cm2。
This study presents a high-performance biofuel cell based on the covalent immobilizing of glucose oxidase (GOx) on chitosan coated carbon cloth as an anodic catalyst. The chitosan was coated by the coagulation of an aqueous solution of chitosan on the carbon cloth surface. The N-(3-dimethylaminopropyl)-N''-ethylcarbodiimide (EDC) and N-hydroxysuccinimide (NHS) was used as coupling agents for GOx immobilization. The response surface methodology (RSM) and Box-Behnken design were employed to search the optimal immobilization conditions and understand the significance of the factors affecting the immobilized GOx activity. The results indicated that the pH, and the enzyme/support ratio are the statistically significant factors for GOx immobilization. In the ridge max analysis, the optimal immobilization conditions include a reaction time of 50 min, a pH of 5.9, and an enzyme/support ratio of 3 (w/w). Under the optimal condition, the predicted and the experimental immobilized GOx activities were 34.42±1.07 and 33.50±0.92 U/g-support, respectively. Based on the regression model, the carbon cloths with various GOx activities were prepared, and the GOx activity effect on the power density generated from the biofuel cell was investigated. The power density was increased with GOx activity, and the maximum power 1.672 mW/cm2 was obtained at a cell voltage of 0.44 V.
其他識別: U0005-0507201216185800
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