Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/40330
標題: Transcription repressor Slug promotes carcinoma invasion and predicts outcome of patients with lung adenocarcinoma
作者: Shih, J.Y.
陳健尉
Tsai, M.F.
Chang, T.H.
Chang, Y.L.
Yuan, A.
Yu, C.J.
Lin, S.B.
Liou, G.Y.
Lee, M.L.
Chen, J.J.W.
Hong, T.M.
Yang, S.C.
Su, J.L.
Lee, Y.C.
Yang, P.C.
關鍵字: epithelial-mesenchymal transitions
e-cadherin expression
human
breast-cancer
zinc-finger gene
hepatocellular-carcinoma
e47
repressors
factors snail
growth-factor
neural crest
cell-line
期刊/報告no:: Clinical Cancer Research, Volume 11, Issue 22, Page(s) 8070-8078.
摘要: Purpose: In a previous genome-wide gene expression profiling analysis using an invasion cancer cell lines model, we have identified Slug as selectively overexpressed in the highly invasive cancer cells. Here, we investigated the clinical significance of Slug in lung adenocarcinoma and the role of Slug in the process of cancer cell invasion and metastasis. Experimental Design: Real-time quantitative reverse transcription-PCR was used to investigate Slug mRNA in surgically resected lung adenocarcinoma of 54 patients and its correlation with survival. We overexpressed Slug in a lung adenocarcinoma cell line with very low Slug levels and investigated the in vitro and in vivo effects of Slug expression. Results: High expression of Slug mRNA in lung cancer tissue was significantly associated with postoperative relapse (P = 0.03) and shorter patient survival (P < 0.001). The overexpression of Slug enhanced xenograft tumor growth and increased microvessel counts in angiogenesis assay. Both inducible and constitutive overexpression of Slug suppressed the expression of E-cadherin and increased the in vitro invasive ability. Zymography revealed increased matrix metalloproteinase-2 activity in Slug overexpressed cells. ELISA, reverse transcription-PCR, and immunohistochemistry confirmed the increase of matrix metalloproteinase-2 proteins and mRNA in Slug overexpressed cells and xenograft tumors. Conclusions: Slug expression can predict the clinical outcome of lung adenocarcinoma patients. Slug is a novel invasion-promoting gene in lung adenocarcinoma.
URI: http://hdl.handle.net/11455/40330
ISSN: 1078-0432
文章連結: http://dx.doi.org/10.1158/1078-0432.ccr-05-0687
Appears in Collections:生物醫學研究所

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