Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/40367
標題: Identification of ESTs differentially expressed in green and albino mutant bamboo (Bambusa edulis) by suppressive subtractive hybridization (SSH) and microarray analysis
作者: Lin, C.S.
陳健尉
Lai, Y.H.
Sun, C.W.
Liu, N.T.
Tsay, H.S.
Chang, W.C.
Chen, J.J.W.
關鍵字: tumor protein tctp
dwarf off-types
arabidopsis-thaliana
cdna
microarray
dna microarray
genes
evolution
transport
membrane
bananas
期刊/報告no:: Plant Cell Tissue and Organ Culture, Volume 86, Issue 2, Page(s) 169-175.
摘要: To gain a better understanding of gene expression in bamboo (Bambusa edulis Murno), we have used a combination of suppressive subtractive hybridization (SSH), microarray hybridization analysis, sequencing, and bioinformatics to identify bamboo genes differentially expressed in a bamboo albino mutant. Ten expressed sequence tags (ESTs) were found to be differentially expressed; these were isolated and sequenced. RT-PCR analysis of these ESTs supported the results of the microarray analysis. Six ESTs that were nucleus-encoded exhibited differential expression patterns in the green wild-type bamboo relative to the albino mutant. These genes (exception being the Rubisco small subunit) were non-photosynthesis-related genes. The development of a specific SSH cDNA library in which most of the chloroplast-encoded or photosynthesis-related genes had been subtracted proved to be useful for studying the function of non-photosynthesis-related genes in the albino bamboo mutants with aberrant chloroplast genome. The combined use of this SSH library with microarray analysis will provide a powerful analytical tool for future studies of the bamboo genome.
URI: http://hdl.handle.net/11455/40367
ISSN: 0167-6857
文章連結: http://dx.doi.org/10.1007/s11240-006-9105-3
Appears in Collections:生物醫學研究所

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