Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/41160
標題: Application of immobilized horseradish peroxidase for the removal of p-chlorophenol from aqueous solution
作者: Lai, Y.C.
林松池
Lin, S.C.
關鍵字: enzyme immobilization
horseradish peroxidase
chlorophenol
waste-water
phenol removal
catalyzed polymerization
enzymatic
removal
precipitation
4-chlorophenol
kinetics
期刊/報告no:: Process Biochemistry, Volume 40, Issue 3-4, Page(s) 1167-1174.
摘要: The development and application of an immobilized horseradish peroxidase (HRP) system with porous aminopropyl glass (APG) beads are reported. The optimal condition for the immobilization of HRP on APG with glutaraladhyde, which leads to a specific activity of 50.7 U/g APG, was identified. Upon immobilization, the operation pH range was broadened downward by one pH unit from 7.5-9.5 to 6.5-9.5 and optimal operation temperature was increased by at least 10degreesC. Thermostability of HRP was also improved by approximately 20% upon immobilization. The application of such HRP immobilized APG for the removal of p-chlorophenol from water was investigated. An HPLC method for the quantification of p-chlorophenol was developed to substitute for spectrophotometric methods that are subject to interferences from proteins and other UV-absrobing compounds in the reaction mixtures. The polymerization of p-chlorophenol into insoluble precipitate was completed within 3 h after the initiation of reaction with the addition of hydrogen peroxide with a maximal removal efficiency of 25%, observed at pH 7.5. The addition of poly(ethylene glycol) significantly enhanced the removal efficiency presumably via the formation of a protective shield in the vicinity of the active site of HRP from the free radicals formed during polymerization and can thus reduce the amount of enzymes needed to achieve the desired removal efficiency. The reusability of the HRP immobilized APG was also demonstrated. (C) 2004 Elsevier B.V. All rights reserved.
URI: http://hdl.handle.net/11455/41160
ISSN: 0032-9592
文章連結: http://dx.doi.org/10.1016/j.procbio.2004.04.009
Appears in Collections:化學工程學系所

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