Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/41169
標題: Characterization and application of PEGylated horseradish peroxidase for the synthesis of poly(2-naphthol)
作者: Jeng, F.Y.
林松池
Lin, S.C.
關鍵字: horseradish peroxidase
poly(ethylene glycol)
PEGylation
naphthol
miscible organic-solvents
salt-induced activation
enzymatic catalysis
nonaqueous media
methoxypolyethylene glycol
chemical modification
polyethylene-glycol
lignin peroxidase
aqueous-solution
water
期刊/報告no:: Process Biochemistry, Volume 41, Issue 7, Page(s) 1566-1573.
摘要: PEGylated horseradish peroxidase (PEG-HRP) was prepared by reacting activated monomethoxy poly(ethylene glycol) with horseradish peroxidase (HRP). Quantitative conversion of HRP to PEG-HRP was observed at a HRP to PEG molar ratio of 1-250 in pH 9.0 borate buffer at 25 degrees C for 3 h. The PEG-HRP conjugates obtained are soluble in various hydrophobic solvents including toluene and benzene. The PEG-HRP conjugates exhibit higher activity than the native HRP with and without the inclusion of equivalent amount of free PEG. Upon conjugation the optimal reaction temperature was increased by 30 degrees C. The PEG-HRP conjugates are relatively active in acetonitrile and dimethylformamide and are capable of catalysing the polymerization of 2-naphthol for the production of fluorescent poly(2-naphthol). The average molecular weight of the polymers, ranging from 900 to 1700, is affected by the concentration of acetonitrile in the reaction medium and is higher than that obtained with the reversed micellar system. The ease of separation of the synthesized polymers from the PEG-HRP conjugates can facilitate the recycling of enzymes. (c) 2006 Elsevier Ltd. All rights reserved.
URI: http://hdl.handle.net/11455/41169
ISSN: 1359-5113
文章連結: http://dx.doi.org/10.1016/j.procbio.2006.02.021
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