Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/41701
標題: Simultaneous purification and immobilization of penicillin G acylase using bifunctional membrane
作者: Chen, C.I.
劉永銓
Chen, C.W.
Huang, C.W.
Liu, Y.C.
關鍵字: penicillin G acylase
immobilized metal affinity membrane
chelated
copper ion bond
multi-covalent bond
bifunctional membrane
metal affinity membranes
multipoint covalent attachment
human
growth-hormone
escherichia-coli
additional stabilization
epoxy
sepabeads
chromatography
proteins
supports
ion
期刊/報告no:: Journal of Membrane Science, Volume 298, Issue 1-2, Page(s) 24-29.
摘要: In this study, the bifunctional membrane (epoxy and immobilized copper ion) was constructed to study the effect on penicillin G acylase (PGA) purification and immobilization. In the procedures, PGA was firstly chelated with the immobilized copper on the membrane at 18 'C, for 12 h. Then, it was covalently bound to the membrane via epoxy group under the incubation conditions (i.e., pH 10.0, 18 degrees C, 76 h). In the construction of bifunctional membrane, iminodiacetic acid disodium (IDA) concentration critically affected the bifunctional group ratio in the membrane. When IDA concentration was higher than 0.500 M, the chelated copper ion in membrane reached its a saturation of 52.2 mu mol/disc. Meanwhile, when IDA concentration was 0.050 M, the chelated copper ion would reach 29.9 mu mol/disc, nearly 58% of its saturation. The membrane prepared by 0.05 M IDA was employed to simultaneously purify and immobilize PGA. The resulting PGA membrane was stable even when the chelated copper ion was eluted with ethylenediaminetetraacetic acid disodium salt dehydrate. It was noted that 96.3% of the PGA activity could be retained under 26 times' reactions within more than 2 months. With this stable property, this immobilization approach could be further developed and applied for industrial processes. (c) 2007 Elsevier B.V. All rights reserved.
URI: http://hdl.handle.net/11455/41701
ISSN: 0376-7388
文章連結: http://dx.doi.org/10.1016/j.memsci.2007.03.054
Appears in Collections:化學工程學系所

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