Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/41706
標題: Optimization of cultivation conditions for the production of chitinase from Paenibacillus sp CHE-N1
作者: Kao, P.M.
劉永銓
Tsai, P.
Liu, Y.C.
Chang, Y.C.
關鍵字: chitinase
response surface methodology
medium optimization
Paenibacillus sp.
response-surface methodology
bacillus-thuringiensis
gluconobacter-suboxydans
trichoderma-harzianum
verticillium-lecanii
serratia-marcescens
tartaric acid
solid-state
fermentation
growth
期刊/報告no:: Journal of the Chinese Institute of Chemical Engineers, Volume 37, Issue 4, Page(s) 355-363.
摘要: A bacterial strain capable of secreting high levels of chitinase was isolated from chitin-enriched soil and identified as Paenibacillus sp. CHE-N1. This strain, which shows high potential as an antagonist against fungi, was grown in a chitin-containing medium to evaluate its ability to produce chitinase. The statistical design method was used to determine the optimal chitinase-producing conditions. Factors, including carbon sources and nitrogen sources in the medium compositions, initial pH, and the temperature of fermentation, were investigated. The obtained optimal conditions were: CSCP 55.4 g/L, peptone 1.8 g/L, and (NH4)(2)SO4 1.6 g/L, with an optimal initial pH and temperature of 8.5 and 343 degrees C, respectively. Under these conditions, the optimal volumetric enzyme activity could reach 8.1 U/mL, about a 4-fold increase as compared to results under basic conditions.
URI: http://hdl.handle.net/11455/41706
ISSN: 0368-1653
Appears in Collections:化學工程學系所

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