Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/41737
DC FieldValueLanguage
dc.contributor.authorLiaw, H.W.en_US
dc.contributor.author蔡毓楨zh_TW
dc.contributor.authorChen, J.M.en_US
dc.contributor.authorTsai, Y.C.en_US
dc.date2006zh_TW
dc.date.accessioned2014-06-06T08:05:49Z-
dc.date.available2014-06-06T08:05:49Z-
dc.identifier.issn1533-4880zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/41737-
dc.description.abstractAn amperometric biosensor for the determination of ethanol has been constructed. It comprises a multiwalled carbon nanotubes (MWNTs) conduit, a Nafion binder, and an alcohol dehydrogenase (ADH) function. The measurement of ethanol is based on the signal produced by beta-nicotinamide adenine dinucleotide (NADH), the product of the enzymatic reaction. The MWNTs are cylindrical with an outer diameter in the range 40-60 nm, an inner diameter in the range 2-5 nm, and a length of up to several micrometers. The homogeneity of the resulting nanobiocomposite film was characterized by field emission scanning electron microscopy (FESEM) and atomic force microscopy (AFM). The performance of the MWNTs-Nafion-ADH nanobiocomposite modified glassy carbon electrode was examined using cyclic voltammetry and amperometry in presence of NADH and in the presence of ethanol. The electrocatalytic activity of MWNTs towards the oxidation of NADH has allowed an effective low-potential amperometric determination of ethanol. In the case of 6 mgmL(-1) ADH, the MWNTs-Nafion-ADH nanobiocomposite film displayed a sensitivity of 830 nAmM(-1), a linear range up to 0.1 mM, a detection limit of 3 mu M, and a response time of about 4 s.en_US
dc.language.isoen_USzh_TW
dc.relationJournal of Nanoscience and Nanotechnologyen_US
dc.relation.ispartofseriesJournal of Nanoscience and Nanotechnology, Volume 6, Issue 8, Page(s) 2396-2402.en_US
dc.relation.urihttp://dx.doi.org/10.1166/jnn.2006.510en_US
dc.subjectmultiwalled carbon nanotubesen_US
dc.subjectalcohol dehydrogenaseen_US
dc.subjectNafionen_US
dc.subjectNADHen_US
dc.subjectethanol biosensoren_US
dc.subjectelectrocatalytic propertiesen_US
dc.subjectmodified electrodesen_US
dc.subjectfield emitteren_US
dc.subjectfilmsen_US
dc.subjectchromatographyen_US
dc.subjecttryptophanen_US
dc.subjectoxidaseen_US
dc.subjectglucoseen_US
dc.subjecturineen_US
dc.titleDevelopment of an amperometric ethanol biosensor based on a multiwalled carbon nanotube-Nafion-alcohol dehydrogenase nanobiocompositeen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1166/jnn.2006.510zh_TW
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