請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/42063
標題: Microalgae cultivation and purification of carotenoids using supercritical anti-solvent recrystallization of CO2 + acetone solution
作者: Wu, Jia-Jiuan
Hong, Siang-En
Wang, Yuan-Chuen
Hsu, Shih-Lan
Chieh-Ming, J.Chang
關鍵字: Microalgae cultivation
Zeaxanthin
Column elution fractionation
Carbon dioxide
Supercritical anti-solvent precipitation
摘要: Zeaxanthin is a type of xanthophyll found in carotenoids, which is produced as a food supplement frequently used for human retinal protection. These works investigated growth rate and harvest of the sea water algal species of Nannochloropsis oculata (N. oculata) by in-door and out-door cultivations; stirred ultrasonic acetone extraction of zeaxanthin from; employed column fractionation to obtain zeaxanthinrich elution; and then coupled it with supercritical carbon dioxide anti-solvent (SAS) precipitation to generate the highly pure submicron-sized zeaxanthin. The ultrasonic extract contained 38.2 mg/g of zeaxanthin and then the column elution fractionation increased the zeaxanthin content to 41% (410.3 mg/g) with a recovery of 82.3%. The SAS precipitation at 215 bar, 50 ◦C for 24 min of injection time enhanced the zeaxanthin content to 58% (582.4 mg/g) with a recovery of 67.2%. Experimental results showed that the amount of zeaxanthin increased with total SAS time and feed concentration had an effect of enlarging the precipitates due to agglomeration and accumulation. The SAS precipitation of the column purified algal solution demonstrated a production of micro/nano-sized lamellar particulates that contained high amounts of zeaxanthin.
URI: http://hdl.handle.net/11455/42063
ISSN: 0896-8446
文章連結: http://dx.doi.org/10.1016/j.supflu.2012.02.020
顯示於類別:化學工程學系所

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