Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/44781
標題: Crystal structure of DFA0005 complexed with alpha-ketoglutarate: A novel member of the ICL/PEPM superfamily from alkali-tolerant Deinococcus ficus
作者: Liao, C.J.
周三和
Chin, K.H.
Lin, C.H.
Tsai, P.S.F.
Lyu, P.C.
Young, C.C.
Wang, A.H.J.
Chou, S.H.
楊秋忠
關鍵字: Deinococcus ficus
organic waste left-over decomposition
alkali-tolerant
ICL/PEPM superfamily
alpha-ketoglutarate ligand
AKG
2-methylisocitrate lyase prpb
protein-protein interactions
petal death
protein
phosphoenolpyruvate mutase
escherichia-coli
mycobacterium-tuberculosis
isocitrate lyase
pep mutase
enzyme
catalysis
期刊/報告no:: Proteins-Structure Function and Bioinformatics, Volume 73, Issue 2, Page(s) 362-371.
摘要: The crystal structure of the DFA0005 protein complexed with alpha-ketoglutarate (AKG) from an alkali-tolerant bacterium Deinococcus ficus has been determined to a resolution Of 1.62 A. The monomer forms an incomplete alpha 7/beta 8 barrel with a protruding alpha 8 helix that interacts extensively with another subunit to form a stable dimer of two complete alpha 8/beta 8 barrels. The dimer is further stabilized by four glycerol molecules situated at the interface. One unique AKG ligand binding pocket per subunit is detected. Fold match using the DALI and SSE servers identifies DFA0005 as belonging to the isocitrate lyase/phosphoenol-pyruvate mutase (ICL/PEPM) superfamily. However, further detailed structural and sequence comparison with other members in this superfamily and with other families containing AKG ligand indicate that DFA0005 protein exhibits considerable distinguishing features of its own and can be considered a novel member in this ICL/PEPM superfamily.
URI: http://hdl.handle.net/11455/44781
ISSN: 0887-3585
文章連結: http://dx.doi.org/10.1002/prot.22071
Appears in Collections:土壤環境科學系

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