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|標題:||Pseudomonas sagittaria sp. nov., a novel siderophore-producing bacterium isolated from oil-contaminated soil|
|摘要:||An aerobic, Gram-negative, rod-shaped bacterium with single polar flagellum, designated as strain CC-OPY-1T, was isolated from an oil-contaminated site in Taiwan. Strain CC-OPY-1T produces siderophores, and can grow at temperature 25-37°C, pH 5.0-9.0 and tolerate <5% (w/v) NaCl. The 16S rRNA gene sequence analysis of strain CC-OPY-1T showed high pairwise sequence similarity to Pseudomonas alcaligenes BCRC 11893T (97.1%), P. alcaliphila DSM 17744T (97.1%), P. tuomuerensis JCM 14085T (97.1%), P. toyotomiensis JCM 15604T (96.9%) and lower sequence similarity to remaining Pseudomonas species. The phylogenetic trees reconstructed based on gyrB and rpoB gene sequences supported the classification of strain CC-OPY-1T as a novel member of the genus Pseudomonas. The predominant quinone system is ubiquinone (Q-9) and the DNA G+C content is 68.4±0.3 mol%. The major fatty acids were C12:0, C16:0, C17:0 cyclo, and summed features 3, and 8 consisting of C16:1 ω7c/C16:1 ω6c and C18:1 ω7c/C18:1 ω6c, respectively. The major polar lipids were phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidylcholine (PC) and two unknown phospholipids (PL1-2). Due to distinct phylogenetic, phenotypic and chemotaxonomic features, strain CC-OPY-1T is proposed to represent a novel species within the genus Pseudomonas for which the name Pseudomonas sagittaria sp. nov. is proposed. The type strain is CC-OPY-1T (=BCRC 80399T =JCM 18195T).|
|Appears in Collections:||土壤環境科學系|
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