請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/44872
標題: Associations of allergenic soybean proteins with piglet skin allergic reaction and application of polyclonal antibodies
作者: Chen, Chen Chi
Lee, Tzu Tai
Hsu, Chin Bin
Huang, Chien Wei
Yu, Bi
關鍵字: fermentation
skin prick test
soybean meal
lactic-acid bacteria
early-weaned pigs
beta-conglycinin
transgenic
rice
globulin
products
meal
immunoreactivity
glycinin
subunit
摘要: A skin prick test was conducted to evaluate the skin allergic reaction of piglets caused by allergenic proteins contained in soybean meal. The data accumulated from subcutaneous piglet skin tissue indicated that allergenic proteins contained in soybean meal crude extracts, even in low dosage levels (7 mu g), caused immunological redness and inflammation within 5 min. The dosages above 200 mu g of beta-conglycinin caused inflammation covering a larger area. The glycinin had less of an influence on skin allergenic reaction dosages than beta-conglycinin did. The antibodies used for beta-conglycinin and glycinin subunits did not exhibit cross-recognition to other subunits or Leguminosae members, such as green beans, lupins and red beans. The polyclonal antibodies further indicated that some allergenic proteins were present after examining soybean meal fermented products individually by Aspergillus or Lactobacillus. None of the allergenic proteins were detected in soybean meal underwent two-stage fermentation. The skin prick test was found to be a convenient method for evaluating the skin allergic reaction of soy allergenic proteins in piglets. The produced polyclonal antibodies are based on subunits of allergenic proteins and can be used to detect the allergenic proteins present in soya products and soybean meal fermented products.
URI: http://hdl.handle.net/11455/44872
ISSN: 1836-0939
顯示於類別:動物科學系

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