Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/44889
標題: Butyrolactone I reversibly alters nuclear configuration, periooplasmic microtubules and development of porcine oocytes
作者: Weng, Y.C.
朱志成
Sha, S.W.
Chiou, C.M.
Tang, P.C.
Yang, J.H.
Ju, J.C.
唐品琦
關鍵字: cdc2 kinase
germinal vesicle
microtubule
oocyte
pig
cyclin-dependent kinases
germinal vesicle stage
pig oocytes
bovine
oocytes
chromosome condensation
meiotic inhibition
vitro maturation
mouse oocytes
cdc2 kinase
roscovitine
期刊/報告no:: Theriogenology, Volume 67, Issue 3, Page(s) 509-519.
摘要: In the present study, we investigated the effects of specific cdc2 kinase inhibitor, butyrolactone I (BL 1) on the prevention of germinal vesicle breakdown, changes of microtubular structures, and development of porcine oocytes after removal of the drug. In Experiment 1, cumulus-oocyte complexes (COCs) were cultured (44 h) in NCSU-23 medium containing different concentrations of BL I. The percentages of oocytes remaining at GV stage were 0, 0, 32, 80, and 84% (P < 0.05), and the maturation rates were 86, 63, 30, 0, and 0% (P < 0.05) for oocytes treated with 0, 10, 20, 40, and 80 mu M of BL I, respectively. When oocytes were released from BL I incubation (Experiment 2) and cultured for an additional 44 h, 79, 84, and 83% of oocytes resumed meiosis, but only 52, 38 and 17% of oocytes reached normal metaphase 11 (MII) stage in the groups treated with 20, 40 and 80 mu M BL 1, respectively. In Experiments 3-5, reversibility and development of oocytes and embryos were evaluated after removal of the inhibitor. A reduced duration of BL I incubation (22 h) at 20 mu M increased the percentage of oocytes remaining at the GV stage compared to the control group (85% versus 9%, P < 0.05). Blastocyst rates were lower in treatment groups than in the control (44 h) group (0-14% versus 24%; P < 0.05). However, all developing blastocysts possessed similar cell numbers, regardless of the drug-treated or non-treated controls. Taken together, treatment with 20-80 mu M of BL I effectively prevented the resumption of meiosis and polymerization of periooplasmic microtubules. Furthermore, reversibility of the oocytes after reduced duration of BL I treatment was satisfactory. (c) 2006 Elsevier Inc. All rights reserved.
URI: http://hdl.handle.net/11455/44889
ISSN: 0093-691X
文章連結: http://dx.doi.org/10.1016/j.theriogenology.2006.08.017
Appears in Collections:動物科學系

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.