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標題: Surgical oocyte retrieval and the developmental potential of the oocytes derived from prepubertal calves
作者: Yeh, S.P.
Ju, J.C.
Tseng, J.K.
Chou, P.C.
Chen, C.H.
Chih, T.
Fan, Y.K.
關鍵字: oocyte
surgical retrieval
in-vitro maturation
matured bovine oocytes
calf oocytes
follicular aspiration
embryo production
期刊/報告no:: Asian-Australasian Journal of Animal Sciences, Volume 17, Issue 2, Page(s) 174-178.
摘要: The objectives of this study were to investigate the ovarian responsiveness of juvenile calves to exogenous gonadotropin treatments and to establish the oocyte retrieval technique for prepubertal heifers. Three 78-day-old calves were treated with 4 doses (40, 30, 30 and 30 mg) of FSH (Folltropin V) at 12 h interval up to 229 day-old. Surgical oocyte retrieval was performed 24 h after the last injection of FSH. Calves with good ovarian responses to FSH treatment had an average ovarian size of 5x3 cm compared to 3x2 cm in the less-responsive animals. Large variations were observed in the number of total follicles (51 45), aspirated follicles (39 +/- 36), oocytes recovered (23 +/- 25) and usable oocytes recovered (11 +/- 19) during 78 to 229 day-old. Oocytes derived from prepubertal calves had significantly lower maturation rate than those from cows (34 vs. 100%, p<0.05). Mean diameters of calf oocytes (144 +/- 1 &mu;m) and ooplasm (110 +/- 1 &mu;m) were significantly lower than those of cows (149 +/- 1 and 125 +/- 1 &mu;m, respectively). The diameter of the ooplasm also increased significantly after in vitro maturation (IVM) (108 +/- 1 vs. 112 +/- 1 &mu;m). However, further studies are required to optimize the IVP system for the oocytes derived from prepubertal heifers.
ISSN: 1011-2367
Appears in Collections:動物科學系



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