Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/44927
標題: Follicular Oocytes Better Support Development in Rabbit Cloning Than Oviductal Oocytes
作者: Sung, L.Y.
朱志成
Chen, C.H.
Xu, J.
Lin, T.A.
Su, H.Y.
Chang, W.F.
Liu, C.C.
Sung, Y.S.
Cheng, W.T.K.
Zhang, J.F.
Tian, X.C.
Ju, J.C.
Chen, Y.E.
Wu, S.C.
Du, F.L.
關鍵字: cell nuclear transfer
maturation-promoting factor
cloned rabbits
in-vitro
fibroblast cells
mouse cloning
cumulus cells
donor cells
embryos
activation
期刊/報告no:: Cellular Reprogramming, Volume 13, Issue 6, Page(s) 503-512.
摘要: This study was conducted to determine the effect of rabbit oocytes collected from ovaries or oviducts on the developmental potential of nuclear transplant embryos. Donor nuclei were obtained from adult skin fibroblasts, cumulus cells, and embryonic blastomeres. Rabbit oocytes were flushed from the oviducts (oviductal oocytes) or aspirated from the ovaries (follicular oocytes) of superovulated does at 10, 11, or 12 h post-hCG injection. The majority of collected oocytes were still attached to the sites of ovulation on the ovaries. We found that follicular oocytes had a significantly higher rate of fusion with nuclear donor cells than oviductal oocytes. There was no difference in the cleavage rate between follicular and oviductal groups, but morula and blastocyst development was significantly higher in the follicular group than in the oviductal group. Two live clones were produced in follicular group using blastomere and cumulus nuclear donors, whereas one live clone was produced in the oviductal group using a cumulus nuclear donor. These results demonstrate that cloned rabbit embryos derived from follicular oocytes have better developmental competence than those derived from oviductal oocytes.
URI: http://hdl.handle.net/11455/44927
ISSN: 2152-4971
文章連結: http://dx.doi.org/10.1089/cell.2011.0030
Appears in Collections:動物科學系

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