Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/44964
DC FieldValueLanguage
dc.contributor.authorTseng, J.K.en_US
dc.contributor.author朱志成zh_TW
dc.contributor.authorTang, P.C.en_US
dc.contributor.authorJu, J.C.en_US
dc.contributor.author唐品琦zh_TW
dc.date2006zh_TW
dc.date.accessioned2014-06-06T08:14:11Z-
dc.date.available2014-06-06T08:14:11Z-
dc.identifier.issn0093-691Xzh_TW
dc.identifier.urihttp://hdl.handle.net/11455/44964-
dc.description.abstractThe precise physiological causes that result in reduced development of oocytes after heat shock (HS) are not clear. In this study, apoptosis, heat shock protein70 (hsp70), and in vitro development of porcine oocytes were evaluated after HS. Porcine cumulus-oocyte complexes (COCs) were subjected to in vitro maturation for 42 It. The matured oocytes were then heated at 41.5 degrees C for 0 h (control, C0h), 1 h (HS1h), 2 It (HS2h), or 4 h (HS4h). An additional group of oocytes was cultured for 4 h without HS (control, C4h). In Experiment 1, expression of hsp70 was detected by Western-blotting and no difference between controls and HS groups was observed. In Experiment 2, apoptosis of matured oocytes after HS was examined by Annexin V-FITC and TUNEL. No significant TUNEL-positive signals were detected in the heated oocytes compared to the controls, but the intensity of Annexin V-FITC labeling among different groups increased with length of HS and in vitro culture (P < 0.05). Oocytes were parthenogenetically activated by an electric pulse plus 6-DMAP (Experiment 3). Mean (+/- S.E.M.) embryonic development in HS2h (cleavage: 42 +/- 29%; blastocyst: 11 +/- 10%) and HS4h (cleavage: 36 +/- 28%; blastocyst: 11 +/- 8%) were decreased when compared to those in C0h (cleavage: 63 +/- 12%; blastocyst: 24 +/- 14%) and C4h (cleavage: 66 +/- 8%; blastocyst: 21 +/- 11%). Numbers of blastocysts with TUNEL-positive signals were similar among groups, but the signals increased before the eight-cell stage in HS groups (P < 0.05). In conclusion, developmental competence of matured pig oocytes was compromised after heat shock, but it was not closely associated with the expression of oocyte hsp70. However, there may be a link between apoptosis and developmental competence of porcine oocytes. (c) 2006 Elsevier Inc. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationTheriogenologyen_US
dc.relation.ispartofseriesTheriogenology, Volume 66, Issue 5, Page(s) 1073-1082.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.theriogenology.2006.03.003en_US
dc.subjectapoptosisen_US
dc.subjectheat shocken_US
dc.subjecthsp70en_US
dc.subjectoocyteen_US
dc.subjectporcineen_US
dc.subjectheat-shock proteinsen_US
dc.subjectpreimplantation bovine embryosen_US
dc.subjectprogrammeden_US
dc.subjectcell-deathen_US
dc.subjectheat-shock-protein-70 synthesisen_US
dc.subjectambient-temperaturesen_US
dc.subjectmolecular chaperonesen_US
dc.subjectdna fragmentationen_US
dc.subjectgenome activationen_US
dc.subjectmouseen_US
dc.subjectembryosen_US
dc.subjectcumulus cellsen_US
dc.titleIn vitro thermal stress induces apoptosis and reduces development of porcine parthenotesen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.theriogenology.2006.03.003zh_TW
Appears in Collections:動物科學系
文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.