Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/44965
標題: The kinase inhibitor indirubin-3 '-oxime prevents germinal vesicle breakdown and reduces parthenogenetic development of pig oocytes
作者: Hsieh, C.H.
朱志成
Tang, P.C.
Chang, W.H.
Weng, Y.C.
Sha, S.W.
Tseng, J.K.
Chang, L.H.
Ju, J.C.
唐品琦
關鍵字: cdc2 kinase
germinal vesicle
indirubin-3 '-oxime
oocyte
resumption
of meiosis
cyclin-dependent kinases
in-vitro maturation
bovine oocytes
butyrolactone-i
mammalian oocytes
selective inhibitor
chemical
inhibitors
meiotic resumption
porcine oocytes
protein-kinases
期刊/報告no:: Theriogenology, Volume 65, Issue 4, Page(s) 744-756.
摘要: Oocytes undergo spontaneous germinal vesicle breakdown (GVBD) after being released from the follicular environment; this potentially prevents manipulation of the oocyte at the germinal vesicle (GV) stage. The objectives of this Study were to investigate the effects of indirubin, a potent cdc2 kinase inhibitor, on GVBD and microtubular structure of porcine oocytes. Cumulus-oocyte-complexes (COCs) were collected from abattoir-derived ovaries and were randomly allocated to different concentrations of indirubin treatments (0, 10, 25, 50, and 100 mu M in Experiment 1 and 0, 50, 75, and 100 mu M in Experiment 2) during 44 h of IVM. The influences on the GVBD, microtubules, and maturation rates, were evaluated using epifluorescence microscopy. The percentages of oocytes remaining at the GV stage were 0, 16, 26, 69, and 85% for oocytes treated with 0, 10, 25, 50, and 100 mu M of indirubin, respectively, which differed among treatment groups (P < 0.05). However, there were no significant differences between the oocytes treated with 75 and 100 mu M (79 and 81%). The cytoplasmic microtubules were fragmented in oocytes maintained at the GV stage and the chromatin became condensed or aggregated. When COCs were incubated with indirubin (50-75 mu M) for 22 h and then transferred to maturation medium for 44 h (Experiments 3-5), the percentages of oocytes reaching the metaphase 11 stage were generally higher than when the COCs were Cultured in the presence of the drug for 44 h (62-65% versus 44-46%). However, the parthenogenetic development of the oocytes in Experiment 6 was reduced significantly in drug-treated oocytes. In Summary, treatment with 50-75 mu M of indirubin effectively prevented GVBD in porcine oocytes, but the developmental competence of the oocytes was compromised. (c) 2005 Elsevier Inc. All rights reserved.
URI: http://hdl.handle.net/11455/44965
ISSN: 0093-691X
文章連結: http://dx.doi.org/10.1016/j.theriogenology.2005.05.054
Appears in Collections:動物科學系

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.