Please use this identifier to cite or link to this item:
|標題:||The kinase inhibitor indirubin-3 '-oxime prevents germinal vesicle breakdown and reduces parthenogenetic development of pig oocytes|
|期刊/報告no：:||Theriogenology, Volume 65, Issue 4, Page(s) 744-756.|
|摘要:||Oocytes undergo spontaneous germinal vesicle breakdown (GVBD) after being released from the follicular environment; this potentially prevents manipulation of the oocyte at the germinal vesicle (GV) stage. The objectives of this Study were to investigate the effects of indirubin, a potent cdc2 kinase inhibitor, on GVBD and microtubular structure of porcine oocytes. Cumulus-oocyte-complexes (COCs) were collected from abattoir-derived ovaries and were randomly allocated to different concentrations of indirubin treatments (0, 10, 25, 50, and 100 mu M in Experiment 1 and 0, 50, 75, and 100 mu M in Experiment 2) during 44 h of IVM. The influences on the GVBD, microtubules, and maturation rates, were evaluated using epifluorescence microscopy. The percentages of oocytes remaining at the GV stage were 0, 16, 26, 69, and 85% for oocytes treated with 0, 10, 25, 50, and 100 mu M of indirubin, respectively, which differed among treatment groups (P < 0.05). However, there were no significant differences between the oocytes treated with 75 and 100 mu M (79 and 81%). The cytoplasmic microtubules were fragmented in oocytes maintained at the GV stage and the chromatin became condensed or aggregated. When COCs were incubated with indirubin (50-75 mu M) for 22 h and then transferred to maturation medium for 44 h (Experiments 3-5), the percentages of oocytes reaching the metaphase 11 stage were generally higher than when the COCs were Cultured in the presence of the drug for 44 h (62-65% versus 44-46%). However, the parthenogenetic development of the oocytes in Experiment 6 was reduced significantly in drug-treated oocytes. In Summary, treatment with 50-75 mu M of indirubin effectively prevented GVBD in porcine oocytes, but the developmental competence of the oocytes was compromised. (c) 2005 Elsevier Inc. All rights reserved.|
|Appears in Collections:||動物科學系|
Show full item record
TAIR Related Article
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.