Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/45110
標題: The expression of pituitary gland genes in laying geese
作者: Yen, C.F.
許振忠
Lin, H.W.
Hsu, J.C.
Lin, C.
Shen, T.F.
Ding, S.T.
關鍵字: laying geese
pituitary gland
prolactin
luteinizing-hormone
gallus-domesticus
turkey prolactin
ovulatory
cycle
chicken
plasma
cdna
radioimmunoassay
protein
cloning
期刊/報告no:: Poultry Science, Volume 85, Issue 12, Page(s) 2265-2269.
摘要: The purpose of this study was to detect differential expression of genes in the pituitary gland in laying geese by suppression subtractive hybridization (SSH). Pituitary glands from prelaying and laying geese were dissected for mRNA extraction. The cDNA from pituitary glands of prelaying geese was subtracted from the cDNA from the pituitary glands of laying geese (forward subtraction); the reverse subtraction was also performed. We screened 384 clones with possible differentially expressed gene fragments by differential screening. Sixty-five clones from the differential screening results were subjected to gene sequencing and further analysis. We found that at least 19 genes were highly expressed in the pituitary glands of laying geese compared with prelaying geese. Among these, 6 genes (including 4 novel genes) were confirmed by virtual Northern analysis. We found that prolactin and visinin-like protein were highly expressed in the pituitary glands of laying geese compared with prelaying geese (P < 0.05). Further investigation is needed to demonstrate specific functions of the novel genes discovered in the current study.
URI: http://hdl.handle.net/11455/45110
ISSN: 0032-5791
Appears in Collections:動物科學系

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